Identification of Novel Seroreactive Antigens in Johne's Disease Cattle by Using the Mycobacterium tuberculosis Protein Array

Clinical and Vaccine Immunology : CVI
John P BannantineVivek Kapur

Abstract

Johne's disease, a chronic gastrointestinal inflammatory disease caused byMycobacterium aviumsubspeciesparatuberculosis, is endemic in dairy cattle and other ruminants worldwide and remains a challenge to diagnose using traditional serological methods. Given the close phylogenetic relationship betweenM. aviumsubsp.paratuberculosisand the human pathogenMycobacterium tuberculosis, here, we applied a whole-proteomeM. tuberculosisprotein array to identify seroreactive and diagnosticM. aviumsubsp.paratuberculosisantigens. A genome-scale pairwise analysis of amino acid identity levels between orthologous proteins inM. aviumsubsp.paratuberculosisandM. tuberculosisshowed an average of 62% identity, with more than half the orthologous proteins sharing >75% identity. Analysis of theM. tuberculosisprotein array probed with sera fromM. aviumsubsp.paratuberculosis-infected cattle showed antibody binding to 729M. tuberculosisproteins, with 58% of them having ≥70% identity toM. aviumsubsp.paratuberculosisorthologs. The results showed that only 4 of the top 40 seroreactiveM. tuberculosisantigens were orthologs of previously reportedM. aviumsubsp.paratuberculosisantigens, revealing the existence of a large number of previously unrecognized cand...Continue Reading

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