Identification of novel serum autoantibodies against EID3 in non-functional pancreatic neuroendocrine tumors

Oncotarget
Koji HontaniSatoshi Hirano

Abstract

Pancreatic neuroendocrine tumors (pNETs) are relatively rare heterogenous tumors, comprising only 1-2% of all pancreatic neoplasms. The majority of pNETs are non-functional tumors (NF-pNETs) that do not produce hormones, and as such, do not cause any hormone-related symptoms. As a result, these tumors are often diagnosed at an advanced stage because patients do not present with specific symptoms. Although tumor markers are used to help diagnosis and predict some types of cancers, chromogranin A, a widely used tumor marker of pNETs, has significant limitations. To identify novel NF-pNET-associated antigens, we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX) and identified five tumor antigens (phosphatase and tensin homolog, EP300-interacting inhibitor of differentiation 3 [EID3], EH domain-containing protein 1, galactoside-binding soluble 9, and BRCA1-associated protein). Further analysis using the AlphaLISA® immunoassay to compare serum antibody levels revealed that antibody levels against the EID3 antigen was significantly higher in the patient group than in the healthy donor group (n = 25, both groups). In addition, higher serum anti-EID3 antibody levels in NF-pNET patients corr...Continue Reading

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Citations

Jan 12, 2020·Seminars in Immunology·Makoto KobayashiSamir M Hanash
Mar 7, 2021·Cancers·Hugo de JongeClaudia Scotti

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Methods Mentioned

BETA
AlphaLISA
dot
dot blots
ELISA
PCR

Software Mentioned

AlphaLISA
GraphPad Prism
BLAST
Basic Local Alignment Search Tool ( BLAST )
GraphPad

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