Identification of novel splice variants of the human CD44 gene

Biochemical and Biophysical Research Communications
Elena VelaMarcos Isamat

Abstract

The CD44 gene contains 10 variable exons (v1-v10) that can be alternatively spliced to generate hundreds of different CD44 protein isoforms, several of which have been implicated in the metastatic spread of tumour cells. Here, we describe a cryptic splice site, in intron 6 of the human CD44 gene, used during mRNA processing. This cryptic splice site is used in conjunction with variable exon 3, or independently from it in the form of a pseudo-exon of 49 bp, which generates a stop codon by frame shift in the contiguous variable exon downstream. This pseudo-exon has been found inserted immediately 3' to any other variable exon from v4 to v10, in the final CD44 mRNA. The implication of this cryptic splice site in haltering CD44 protein translation is questioned in the context of Nonsense Mediated Decay and the overall regulation of CD44 expression.

References

Jul 17, 1998·The American Journal of Pathology·X RocaM Isamat
Oct 20, 1998·The American Journal of Pathology·S GoodisonD Tarin
Jul 20, 1999·Experimental and Molecular Pathology·A Herrera-Gayol, S Jothy
Sep 2, 1999·Human Molecular Genetics·P A Frischmeyer, H C Dietz
Aug 1, 2000·Trends in Biochemical Sciences·C W Smith, J Valcárcel
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Helmut PontaPeter A Herrlich
Sep 2, 2004·Journal of Molecular Histology·R Marhaba, M Zöller
Jan 14, 2005·Genes, Chromosomes & Cancer·Zane KalninaAija Linē
Jan 20, 2005·Gene·Stefan StammHermona Soreq
Aug 24, 2005·Nature Structural & Molecular Biology·Jayanthi P GudikoteMiles F Wilkinson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.