Identification of novel staphylococcal virulence genes by in vivo expression technology

Molecular Microbiology
A M LoweR L Deresiewicz

Abstract

We have applied in vivo expression technology (IVET) to the study of staphylococcal virulence. Using a promoter trap that relies on genetic recombination as a reporter of gene expression, we identified 45 staphylococcal genes that are induced during infection in a murine renal abscess model. Of these, only six were known previously; 11 others have homology to known non-staphylococcal genes. The known staphylococcal genes include agrA, part of a key locus regulating numerous virulence products, and a glycerol ester hydrolase, which may enhance staphylococcal survival in abscesses. We constructed 11 strains containing mutations in previously unknown ivi genes. Of these strains, seven were significantly attenuated in virulence compared with the wild-type parent. The mutagenized ivi genes may encode novel staphylococcal virulence factors.

References

Oct 1, 1992·Journal of Medical Microbiology·H D Engler, F A Kapral
Oct 1, 1992·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·A L PanlilioW J Martone
May 1, 1992·Journal of Medical Microbiology·T R ShryockF A Kapral
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D M RanelliS A Khan
Aug 30, 1995·Molecular & General Genetics : MGG·R P NovickS Moghazeh
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·M J MahanJ J Mekalanos
Nov 1, 1994·The Journal of Clinical Investigation·A L CheungA S Bayer
May 15, 1994·FEMS Microbiology Letters·T J Foster, D McDevitt
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·A CamilliJ J Mekalanos
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·L JanosiA Kaji
Jun 1, 1994·Infection and Immunity·R L DeresiewiczD L Kasper
Jul 1, 1996·The Journal of Infectious Diseases·S A HienzJ I Flock
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·J WangS Jin
Nov 1, 1995·Molecular Microbiology·A Camilli, J J Mekalanos
Apr 1, 1996·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·J M Boyce
Jan 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G A Ayliffe
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M HeithoffM J Mahan

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Citations

Apr 15, 2004·Current Opinion in Biotechnology·Willem M de VosMichiel Kleerebezem
Jul 22, 1999·Veterinary Microbiology·A LammersF G van Zijderveld
Jun 24, 2000·FEMS Immunology and Medical Microbiology·L ZhangL Frykberg
Jul 12, 2002·FEMS Immunology and Medical Microbiology·James BurnieRuth Matthews
Jan 16, 1999·Current Opinion in Biotechnology·N J Saunders, E R Moxon
Nov 5, 1999·Trends in Microbiology·M O Clements, S J Foster
Jan 19, 2000·Trends in Microbiology·I Msadek
Oct 26, 2000·Current Opinion in Microbiology·J E SheaR G Feldman
Aug 18, 2000·Immunology and Cell Biology·J A Triccas, B Gicquel
Feb 24, 2001·Environmental Microbiology·P B Rainey
Jun 21, 2011·Omics : a Journal of Integrative Biology·Fabio BagnoliRino Rappuoli
May 18, 2007·Molecular Plant-microbe Interactions : MPMI·Xue-Xian Zhang, Paul B Rainey
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H Smith
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D S Merrell, A Camilli
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K E Unsworth, D W Holden
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D M HeithoffM J Mahan
Dec 8, 2009·Applied and Environmental Microbiology·Elena StaryRalph Bertram
Jan 1, 2008·Applied and Environmental Microbiology·Martina LeibigRalph Bertram
Feb 22, 2012·Applied and Environmental Microbiology·Huruma Nelwike TuntufyeBruno Maria Goddeeris
Jul 6, 2000·Applied and Environmental Microbiology·S W Lee, D A Cooksey
Oct 5, 2002·Clinical Microbiology Reviews·Mark E Shirtliff, Jon T Mader
Oct 24, 2006·Infection and Immunity·Andrea HamiltonAmanda L Jones
Apr 18, 2000·Infection and Immunity·A P Teixeira-GomesM S Zygmunt

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