Identification of Novel Target for Osteosarcoma by Network Analysis

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Li-Qiang ZhiJian-Bing Ma

Abstract

BACKGROUND Osteosarcoma (OS) is a highly complicated bone cancer involving imbalance of signaling transduction networks in cells. Development of new anti-osteosarcoma drugs is very challenging, mainly due to lack of known key targets. MATERIAL AND METHODS In this study, we attempted to reveal more promising targets for drug design by "Target-Pathway" network analysis, providing the new therapeutic strategy of osteosarcoma. The potential targets used for the treatment of OS were selected from 4 different sources: DrugBank, TCRD database, dbDEMC database, and recent scientific literature papers. Cytoscape was used for the establishment of the "Target-Pathway" network. RESULTS The obtained results suggest that tankyrase 2 (TNKS2) might be a very good potential protein target for the treatment of osteosarcoma. An in vitro MTT assay proved that it is an available option against OS by targeting the TNKS2 protein. Subsequently, cell cycle and apoptosis assay by flow cytometry showed the TNKS2 inhibitor can obviously induce cell cycle arrest, apoptosis, and mitotic cell death. CONCLUSIONS Tankyrase 2 (TNKS2), a member of the multifunctional poly(ADP-ribose) polymerases (PARPs), could be a very useful protein target for the treatment of...Continue Reading

Citations

Aug 14, 2019·Expert Opinion on Emerging Drugs·Claudia Maria HattingerMassimo Serra
Feb 23, 2020·Journal of Orthopaedic Surgery and Research·Kai GaoChaoliang Lv

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Methods Mentioned

BETA
surgical resection
flow cytometry

Software Mentioned

Cytoscape
DAVID
CellQuest
FlowJo
Database for Annotation , Visualization , and Integrated Disco...
Tarbase
Origin8

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis