PMID: 11310559Apr 20, 2001Paper

Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
J FreireM Freire

Abstract

Prothymosin alpha (ProT alpha) is a nuclear protein that is widely distributed in mammalian tissues, and is thought to play a role in cell proliferation. In an attempt to shed light on this role, affinity chromatography on ProT alpha-Sepharose columns was used to identify proteins in subcellular extracts of transformed human lymphocytes (NC37 cells) that interact with ProT alpha in vitro, and thus may interact with ProT alpha in vivo. Immunoblotting techniques were used to screen the ProT alpha-binding fractions for histones and other proteins involved in nuclear transport and cell-cycle control. The most abundant ProT alpha-binding proteins were histones H2A, H2B, H3, and H4. Of the nuclear-transport proteins, karyopherin beta1, Rch-1, Ran, and RCC1 were detected at high concentrations; NTF2, nucleoporin p62, and Hsp70 were detected at low concentrations; while tranportin, CAS, and Ran BPI were not detected. Of the cell-cycle control proteins, PCNA, Cdk2, and cyclin A were detected at high concentrations; cdc2, Cdk4, and cyclin B were detected at very low concentrations; while cyclin D1, cyclin D3, Cip1, and Kip1 were not detected. These results suggest (i) that ProT alpha is transported into the nucleus by the karyopherin bet...Continue Reading

Citations

Apr 21, 2005·Kidney International·Vincent H Gattone
Oct 28, 2010·Biochimica Et Biophysica Acta·Cristina Díaz-JullienManuel Freire
Jun 30, 2007·Annals of the New York Academy of Sciences·Arevik MosoianMary Klotman
Jan 30, 2013·Journal of Cellular Physiology·Diana FerraraSergio Minucci
Jan 28, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Alicia BáezÁlvaro Urbano-Ispizua

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

© 2021 Meta ULC. All rights reserved