Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array

Science in China. Series C, Life Sciences
Jun-kui AiYing-lu Guo

Abstract

To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon membrane and the over-expressed genes were then screened by hybridizing the filter with radioactively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank database. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular endothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohist...Continue Reading

Citations

Mar 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shengquan HuangDianqi Xin
Apr 17, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bing ChenYing Tong
Sep 18, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guofeng ZhangXianghua Yi

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