PMID: 9425168Feb 14, 1998Paper

Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration

The Journal of Cell Biology
L A CaryJ L Guan

Abstract

Previously we have demonstrated that focal adhesion kinase (FAK)-promoted migration on fibronectin (FN) by its overexpression in CHO cells is dependent on FAK autophosphorylation at Y397 and subsequent binding of Src to this site. In this report, we have examined the role of FAK association with Grb2 and p130(Cas), two downstream events of the FAK/Src complex that could mediate integrin-stimulated activation of extracellular signal-regulated kinases (Erks). We show that a Y925F FAK mutant was able to promote cell migration as efficiently as FAK and that the transfected FAK demonstrated no detectable association with Grb2 in CHO cells. In contrast, cells expressing a FAK P712/715A mutant demonstrated a level of migration comparable to that of control cells. This mutation did not affect FAK kinase activity, autophosphorylation, or Src association but did significantly reduce p130(Cas) association with FAK. Furthermore, FAK expression in CHO cells increased tyrosine phosphorylation of p130(Cas) and its subsequent binding to several SH2 domains, which depended on both the p130(Cas) binding site and the Src binding site. However, we did not detect increased activation of Erks in cells expressing FAK, and the MEK inhibitor PD98059 di...Continue Reading

References

Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·T R Polte, S K Hanks
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
May 1, 1995·Molecular and Cellular Biology·M D Schaller, J T Parsons
Jul 1, 1995·Molecular Biology of the Cell·M YebraD A Cheresh
Mar 1, 1995·Molecular Biology of the Cell·X Zhu, R K Assoian
Jul 14, 1995·The Journal of Biological Chemistry·H C ChenJ L Guan
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Apr 14, 1995·Science·E A Clark, J S Brugge
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H C Chen, J L Guan
Apr 12, 1996·The Journal of Biological Chemistry·T NakamotoH Hirai
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Aug 1, 1996·The Journal of Cell Biology·S M FrischP Y Chan-Hui
Dec 1, 1996·The Journal of Cell Biology·J E HungerfordC A Otey
Apr 21, 1997·The Journal of Cell Biology·R L KlemkeD A Cheresh
Jul 1, 1997·Molecular and Cellular Biology·T NakamotoH Hirai

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Citations

Sep 29, 2000·International Journal of Cancer. Journal International Du Cancer·S van der FlierM Dowsett
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T GendaS Hirohashi
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Masashi KohnoShigeru Fujita
Nov 26, 2009·Cellular and Molecular Life Sciences : CMLS·Nadezhda TikhmyanovaErica A Golemis
Nov 13, 2004·Breast Cancer Research and Treatment·Reju KorahRobert Wieder
Jan 27, 2009·Cancer Metastasis Reviews·Jihe Zhao, Jun-Lin Guan
Sep 5, 2006·Reviews in Endocrine & Metabolic Disorders·Angela Bruzzaniti, Roland Baron
Jun 18, 2002·European Journal of Pharmacology·Julius LeytonTerry W Moody
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
Apr 21, 1999·Experimental Hematology·J P Lévesque, P J Simmons
Oct 29, 2002·Cellular Signalling·Mousumi MajumdarWilliam B Stallcup
Oct 2, 2003·Current Opinion in Cell Biology·Kris A DeMaliKeith Burridge
Apr 21, 1999·Current Opinion in Cell Biology·S M Schoenwaelder, K Burridge
Aug 26, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R J JonesM C Frame
Apr 16, 1998·Current Biology : CB·F LiuJ Chernoff
Feb 17, 2000·Trends in Cell Biology·G M O'NeillE A Golemis
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Silvia M GoicoecheaChuanyue Wu
Jan 27, 2004·Nature Cell Biology·Donna J WebbAlan F Horwitz
Aug 3, 2005·Nature Reviews. Cancer·Gordon W McLeanMargaret C Frame
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
May 13, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·L P WengQ Yu
Feb 24, 2001·Cellular Microbiology·C L WeidowA H Bouton
Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Vinicio CarloniMassimo Pinzani
Dec 10, 1999·The Journal of Biological Chemistry·R H PalmerT Hunter
Jul 17, 2009·The Journal of Biological Chemistry·Désirée SpieringStephan Ludwig
Feb 18, 2012·Tissue Engineering. Part B, Reviews·Brandon D RiehlJung Yul Lim

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