Identification of p53 mutations in archival prostate tumors. Sensitivity of an optimized single-strand conformational polymorphism (SSCP) assay

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
X B ShiP H Gumerlock

Abstract

To correlate molecular changes with clinical information in prostate tissue, it is necessary to have accurate methods for screening for mutations in clinically available specimens. We have refined the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis for detection of p53 mutations in routine pathology specimens. These improvements help overcome technical barriers that interfere with SSCP analysis of archival tissues when only small amounts of poorly preserved formalin-fixed DNA are available. Furthermore, prostate samples are heterogeneous in containing tumor, normal tissue, and hyperplastic tissue. To address the first issue, the method included an initial selection of PCR products using exonuclease I, followed by a second-step selection using nested PCR. This step ensures adequate amplification of the target sequence while minimizing artifactual products that could otherwise interfere with mutation analysis. For the second issue, in addition to morphologic selection of appropriate tissue areas, we improved the sensitivity of detection of mutations by using restriction enzyme digestion of products prior to SSCP analysis. Detection of mutations in heterogeneous tissue was evaluated by determi...Continue Reading

References

Oct 1, 1992·Genetic Analysis, Techniques and Applications·T A SmithP J Parry
Oct 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Oren
Aug 21, 1992·Cell·B Vogelstein, K W Kinzler
Jul 5, 1991·Science·M HollsteinC C Harris
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G C Burmer, L A Loeb
Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Jun 15, 1994·Journal of the National Cancer Institute·S G ChiP H Gumerlock
Oct 20, 1993·Journal of the National Cancer Institute·N M NavoneC J Conti
Feb 1, 1994·The Journal of Urology·H J VoellerE P Gelmann
Apr 1, 1993·Genes & Development·C Prives, J J Manfredi
May 15, 1993·Lancet·D A Carson, J M Ribeiro
Jan 1, 1993·The Prostate·A G CarrollE P Gelmann
Aug 1, 1993·PCR Methods and Applications·X B ShiS J DeNardo
Jan 1, 1993·Human Mutation·K Hayashi, D W Yandell
Jan 1, 1994·CA: a Cancer Journal for Clinicians·C C BoringS Montgomery
Mar 1, 1994·International Journal of Cancer. Journal International Du Cancer·W N DinjensJ Trapman
Jan 1, 1993·The Prostate·P J Van VeldhuizenR L Stephens
Feb 1, 1993·Cancer·R Bookstein, D C Allred
Feb 1, 1993·PCR Methods and Applications·W C KusserB W Glickman
Jul 1, 1993·The Journal of Urology·P J EffertE T Liu

❮ Previous
Next ❯

Citations

May 28, 2002·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hiroyoshi SuzukiHidehiro Takei
Jan 23, 2003·Cancer Biotherapy & Radiopharmaceuticals·Xu-Bao ShiRalph W deVere White
Jul 5, 2005·Surgical Oncology·S R J BottM Williamson
Aug 14, 1999·Arthritis and Rheumatism·F KullmannU Müller-Ladner
Feb 27, 1999·Endocrine Reviews·E RuijterJ Schalken

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.