Identification of pactolus, an integrin beta subunit-like cell-surface protein preferentially expressed by cells of the bone marrow.

The Journal of Biological Chemistry
Y ChenJ J Weis

Abstract

We have sought to develop methodologies to identify genes that are preferentially expressed during the differentiation of mast cells from their hematopoietic stem cell precursors. By using a modified differential display protocol, we compared a subset of transcripts expressed in bone marrow cells differentiated into immature mast cells with the exogenous addition of stem cell factor (SCF) or interleukin 3. One gene was identified that was preferentially expressed in the SCF-derived cells and encodes a novel murine integrin beta subunit-like molecule, dubbed Pactolus-1 (Pactolus). Two distinct forms of Pactolus mRNA were detected which, via alternative splicing, are predicted to encode a membrane-bound form and truncated version of the protein. The full-length Pactolus gene product is very similar to a number of beta subunit integrin chains, particularly beta2, with the notable exceptions of the apparent deletion of the metal-binding site within the putative metal ion-dependent adhesion site-like domain of the Pactolus gene product and a cytoplasmic domain that shares no obvious homology to similar domains of the other beta subunit integrin proteins. Although the Pactolus sequence was first identified in immature mast cell sampl...Continue Reading

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Citations

Mar 17, 2001·Journal of Immunological Methods·M AliJ D Isaacs
Feb 6, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K RoundyJ H Weis
May 25, 2007·Proteins·Mehmet Sen, Glen B Legge
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sean GarrisonJohn H Weis
Dec 17, 2020·Cells·Joanna PastwińskaJarosław Dastych

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