Identification of Pantoea stewartii subsp. stewartii by PCR and Strain Differentiation by PFGE

Plant Disease
David CoplinK Geider

Abstract

Stewart's bacterial wilt and leaf blight of sweet corn and maize is caused by Pantoea stewartii subsp. stewartii. This bacterium can be seed transmitted at a low frequency, so it is subject to quarantine restrictions by many countries. To develop a polymerase chain reaction assay for the identification of this pathogen from field samples and for use in seed health tests, four primer pairs were tested. These were selected from the sequences of hrpS, cpsDE, and the 16S rRNA intergenic transcribed spacer (ITS) region. Under optimal reaction conditions, about 20 and 200 cells of P. stewartii could be detected in pure cultures and leaf lesions, respectively. Other plant-associated enteric bacteria (e.g., P. agglomerans pv. herbicola, P. ananas, Erwinia amylovora, and E. carotovora) either did not produce amplicons or they were not the correct size for P. stewartii. To test further for possible false positives, 29 yellow-pigmented bacteria, mainly other Pantoea spp., were isolated from lesions on old corn leaves and assayed with the ITS primer sets. Except for weak, variable reactions with three P. ananas strains, the bacteria did not test positive. Pulsed field gel electrophoresis (PFGE) was evaluated as an additional test to confir...Continue Reading

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Citations

Mar 30, 2010·Applied and Environmental Microbiology·Linda K DickDonald M Stoeckel
Feb 28, 2016·Applied and Environmental Microbiology·Orin C ShanksMano Sivaganesan
Jun 3, 2017·Genome Announcements·Duy An DuongRoderick V Jensen
Jul 10, 2012·Applied and Environmental Microbiology·Valdir R CorreaMargaret G Redinbaugh
Jan 20, 2017·Nucleic Acids Research·Elena Asensio-JuanMarian A Martínez-Balbás
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
Jun 26, 2015·FEMS Microbiology Reviews·Alyssa M Walterson, John Stavrinides
Jul 6, 2018·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (EFSA PLH Panel)David Caffier
Apr 10, 2018·The Plant Pathology Journal·Kwang Yeol BaekSeon-Woo Lee

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