Identification of papain-like cysteine proteases from the bovine piroplasm Babesia bigemina and evolutionary relationship of piroplasms C1 family of cysteine proteases

Experimental Parasitology
Tiago M MartinsAna Domingos

Abstract

Papain-like cysteine proteases have been shown to have essential roles in parasitic protozoa and are under study as promising drug targets. Five genes were identified by sequence similarity search to be homologous to the cysteine protease family in the ongoing Babesia bigemina genome sequencing project database and were compared with the annotated genes from the complete bovine piroplasm genomes of Babesia bovis, Theileria annulata, and Theileria parva. Multiple genome alignments and sequence analysis were used to evaluate the molecular evolution events that occurred in the C1 family of cysteine proteases in these piroplasms of veterinary importance. BbiCPL1, one of the newly identified cysteine protease genes in the B. bigemina genome was expressed in Escherichia coli and shows activity against peptide substrates. Considerable differences were observed in the cysteine protease family between Babesia and Theileria genera, and this may partially explain the diverse infection mechanisms of these tick-borne diseases.

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Citations

Apr 7, 2012·Journal of Proteomics·Isabel MarcelinoAna Varela Coelho
Oct 13, 2015·Ticks and Tick-borne Diseases·Tamara CarlettiMonica Florin-Christensen
Mar 13, 2016·Journal of Molecular Modeling·Potshangbam Angamba MeeteiVaibhav Vindal
Oct 12, 2011·Acta Tropica·Tiago M MartinsAna Domingos
Aug 12, 2017·Current Medicinal Chemistry·Rafael Pinto VieiraRafaela Salgado Ferreira
Apr 21, 2018·Veterinary Sciences·Mariano E AscencioLeonhard Schnittger

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