Identification of Pax3 and Zic1 targets in the developing neural crest

Developmental Biology
Chang-Joon BaeJean-Pierre Saint-Jeannet

Abstract

The neural crest (NC) is a multipotent population of migratory cells unique to the vertebrate embryo, contributing to the development of multiple organ systems. Transcription factors pax3 and zic1 are among the earliest genes activated in NC progenitors, and they are both necessary and sufficient to promote NC fate. In order to further characterize the function of these transcription factors during NC development we have used hormone inducible fusion proteins in a Xenopus animal cap assay, and DNA microarray to identify downstream targets of Pax3 and Zic1. Here we present the results of this screen and the initial validation of these targets using quantitative RT-PCR, in situ hybridization and morpholinos-mediated knockdown. Among the targets identified we found several well-characterized NC-specific genes, including snail2, foxd3, gbx2, twist, sox8 and sox9, which validate our approach. We also obtained several factors with no known function in Xenopus NC, which represent novel regulators of NC fate. The comprehensive characterization of Pax3 and Zic1 targets function in the NC gene regulatory network, are essential to understanding the mechanisms regulating the emergence of this important cell population.

References

Apr 1, 1978·Experimental Cell Research·W D Stuart, D L Porter
Sep 1, 1995·Developmental Biology·P J Kolm, H L Sive
Dec 29, 1995·The Journal of Biological Chemistry·D CommuniJ M Boeynaems
Jan 27, 1998·Developmental Biology·B Christen, J M Slack
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·N MatsukawaO Smithies
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·J JaubertJ L Guénet
Aug 15, 2000·Human Molecular Genetics·N BondurandM Goossens
Nov 25, 2000·Mechanisms of Development·K NakataK Mikoshiba
Apr 6, 2001·European Journal of Pharmacology·N Suarez-HuertaB Robaye
Apr 2, 2003·Human Molecular Genetics·Deborah Lang, Jonathan A Epstein
Oct 3, 2003·Development·Martin Cheung, James Briscoe
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sue KenwrickNancy Papalopulu
May 7, 2004·Development·Martina NagelRudolf Winklbauer
Sep 11, 2004·Development·Nicole M Le DouarinElisabeth Dupin
Feb 5, 2005·Developmental Cell·Anne-Hélène Monsoro-BurqRichard Harland
Mar 16, 2007·Differentiation; Research in Biological Diversity·Yukiyo YamamotoMichael Oelgeschläger
Apr 6, 2007·Molecular Biology of the Cell·Chang-Soo Hong, Jean-Pierre Saint-Jeannet
Jun 8, 2007·Development Genes and Evolution·O J BronchainM Wegnez
Jul 16, 2008·The Journal of Biological Chemistry·Justin S WeinbaumRobert P Mecham
Aug 9, 2008·Developmental Biology·Caroline E HaldinElizabeth A Jones
Oct 4, 2008·Developmental Biology·Byung-Yong Park, Jean-Pierre Saint-Jeannet
Nov 11, 2008·Development·Chang-Soo HongJean-Pierre Saint-Jeannet
Feb 24, 2009·Cellular and Molecular Life Sciences : CMLS·P Moffatt, G P Thomas
May 12, 2009·The Biochemical Journal·Stéphanie CharrinEric Rubinstein
Jan 8, 2011·Developmental Biology·Hua PanCharles P Emerson
Jan 22, 2011·Journal of Neurochemistry·Kathryn L McCabe, Marianne Bronner
Sep 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carol H YanSiew-Lan Ang
Sep 8, 2011·Journal of Cellular Biochemistry·Clarissa S CraftRobert P Mecham
May 2, 2012·Cellular and Molecular Life Sciences : CMLS·Timothy J Stuhlmiller, Martín I García-Castro
May 30, 2012·Developmental Biology·Susanne KlingelDietmar Gradl
Sep 17, 2013·Developmental Biology·Charis L HimedaCharles P Emerson

❮ Previous
Next ❯

Citations

Dec 17, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xing GuHou-Zhong Ding
Sep 16, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Elisabeth Dupin, Nicole M Le Douarin
Jun 24, 2015·Nature Communications·Maria Belen JaurenaJean-Pierre Saint-Jeannet
Jan 15, 2015·Scientific Reports·Chang-Joon BaeJean-Pierre Saint-Jeannet
Mar 13, 2014·Developmental Neurobiology·Chang-Soo HongJean-Pierre Saint-Jeannet
Mar 3, 2018·Epigenetics : Official Journal of the DNA Methylation Society·Carl BaribaultMelanie Ehrlich
May 4, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Crystal D Rogers, Shuyi Nie
Nov 18, 2014·Genesis : the Journal of Genetics and Development·Chang-Soo Hong, Jean-Pierre Saint-Jeannet
Apr 3, 2019·EMBO Reports·Hanna PolevoyDale Frank
Mar 13, 2019·Development·Heather C EtcheversNicole M Le Douarin
Mar 4, 2019·Journal of Molecular Neuroscience : MN·Xia LiangSun Caixing
Nov 1, 2017·Genesis : the Journal of Genetics and Development·Chang-Soo Hong, Jean-Pierre Saint-Jeannet
Apr 3, 2019·Frontiers in Psychology·Amy Niego, Antonio Benítez-Burraco
Jan 8, 2015·Development·Marcos Simões-Costa, Marianne E Bronner
Apr 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nan WangYanfang Gu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.