Identification of peptaibols from Trichoderma virens and cloning of a peptaibol synthetase.

The Journal of Biological Chemistry
Aric WiestCharles M Kenerley

Abstract

The fungus Trichoderma virens is a ubiquitous soil saprophyte that has been applied as a biological control agent to protect plants from fungal pathogens. One mechanism of biocontrol is mycoparasitism, and T. virens produces antifungal compounds to assist in killing its fungal targets. Peptide synthetases produce a wide variety of peptide secondary metabolites in bacteria and fungi. Many of these are known to possess antibiotic activities. Peptaibols form a class of antibiotics known for their high alpha-aminoisobutyric acid content and their synthesis as a mixture of isoforms ranging from 7 to 20 amino acids in length. Here we report preliminary characterization of a 62.8-kb continuous open reading frame encoding a peptaibol synthetase from T. virens. The predicted protein structure consists of 18 peptide synthetase modules with additional modifying domains at the N- and C-termini. T. virens was shown to produce a mixture of peptaibols, with the largest peptides being 18 residues. Mutation of the gene eliminated production of all peptaibol isoforms. Identification of the gene responsible for peptaibol production will facilitate studies of the structure and function of peptaibol antibiotics and their contribution to biocontrol ...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·I L KarleP Balaram
Jan 1, 1991·Progress in Biophysics and Molecular Biology·M S Sansom
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1989·International Journal of Peptide and Protein Research·S RebuffatB Bodo
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Oct 1, 1987·The Journal of Applied Bacteriology·W C JenA Taylor
Jun 28, 1996·The Journal of Biological Chemistry·T SteinH R Morris
Jan 1, 1997·Advances in Microbial Physiology·R Zocher, U Keller
Aug 1, 1997·Chemistry & Biology·M A Marahiel
Mar 21, 1998·Fungal Genetics and Biology : FG & B·J M Baek, C M Kenerley
Feb 18, 1999·Chemistry & Biology·D Konz, M A Marahiel
Jul 28, 1999·Chemistry & Biology·T StachelhausM A Marahiel
Oct 27, 2001·Applied and Environmental Microbiology·S E WilhiteD C Straney
Feb 20, 2002·Chemical Reviews·Mohamed A. MarahielHenning D. Mootz

❮ Previous
Next ❯

Citations

Oct 12, 2012·Journal of Computer-aided Molecular Design·Ammar AbdoMaude Pupin
Dec 3, 2013·Indian Journal of Microbiology·Mala MukherjeeSusanne Zeilinger
Feb 6, 2004·Fungal Genetics and Biology : FG & B·María J PozoCharles M Kenerley
Dec 3, 2005·Nature Reviews. Microbiology·Nancy P KellerJoan W Bennett
Sep 28, 2007·Natural Product Reports·Juliana F de S Daniel, Edson Rodrigues Filho
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Arush ChhabraRajesh S Gokhale
Dec 3, 2010·The Journal of Biological Chemistry·Prasun K MukherjeeCharles M Kenerley
Aug 13, 2011·Molecular Plant-microbe Interactions : MPMI·R Velazquez-RobledoA Herrera-Estrella
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Slavica DjonovićCharles M Kenerley
Mar 5, 2005·Applied and Environmental Microbiology·Susanne ZeilingerRobert L Mach
Aug 10, 2005·Canadian Journal of Microbiology·Xianying WeiDavid C Straney
Aug 7, 2013·Annual Review of Phytopathology·Prasun K MukherjeeCharles M Kenerley
Jan 27, 2010·BMC Evolutionary Biology·Kathryn E Bushley, B Gillian Turgeon
May 18, 2007·BMC Evolutionary Biology·Christian RauschDaniel H Huson
Feb 1, 2014·Genome Biology and Evolution·Bin-Bin XieXiao-Yan Song
Jan 5, 2014·TheScientificWorldJournal·Xu-Ping ShentuChuan-Xi Zhang
Apr 19, 2008·Mycopathologia·Mark A Weaver, Charles M Kenerley
Dec 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Thomas DegenkolbUdo Gräfe
Dec 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Kathrin ReiberTorsten Schwecke
Dec 22, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anne-Isaline Van BohemenYves François Pouchus
Nov 7, 2006·Journal of Microbiological Methods·Sébastien Massart, Haissam M Jijakli
Nov 1, 2007·Molecular Plant Pathology·Ada ViterboCharles Kenerley
Feb 10, 2006·Fungal Genetics and Biology : FG & B·J A VizcaínoE Monte
Oct 15, 2011·Microbiology·Prasun K MukherjeeCharles M Kenerley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.