PMID: 9532590Apr 9, 1998Paper

Identification of peptides binding to IgG in the CSF of multiple sclerosis patients

Multiple Sclerosis : Clinical and Laboratory Research
I CorteseR Cortese

Abstract

Phage displayed random peptide libraries were screened in order to identify phagotopes reacting with the IgG present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. Six families of phagotopes each composed of different isolates were identified. These phagotopes were used as reagents, to characterise IgG present in the CSF of MS patients. The following results were obtained: (a) CSF antibodies from different patients display different specificities; (b) anti-phagotope antibodies are also present in the serum of MS patients; (c) Anti-phagotope antibodies are equally frequent in the serum of MS patients and of healthy individuals; (d) some of the anti-phagotope antibodies are enriched in the CSF of MS patients. These data show that the natural antigen(s) recognised by CSF antibodies is rather common in the general population. By using the selected phagotopes as immunogens in rabbits, we have derived large quantities of anti-phagotope antisera which can provide for useful tools toward the identification of the natural antigen(s) recognised by MS CSF antibodies.

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Citations

Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veerle SomersPiet Stinissen
Oct 16, 2007·Proteomics. Clinical Applications·Stella Elkabes, Hong Li
Mar 7, 2006·Biopolymers·Maria Claudia Alcaro, Anna Maria Papini
Nov 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·John T BallewPatrick S Daugherty
Apr 23, 2008·Current Protocols in Neuroscience·A Bradbury

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