Oct 29, 2018

Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies

BioRxiv : the Preprint Server for Biology
Pradeep Sundaram RajendranKalyanam Shivkumar


Heart rate is under the precise control of the autonomic nervous system. However, the wiring of peripheral neural circuits that regulate heart rate is poorly understood. Here, we developed a clearing-imaging-analysis pipeline to visualize innervation of intact hearts in 3D and employed a multi-technique approach to map parasympathetic and sympathetic neural circuits that control heart rate in mice. We anatomically and functionally identify cholinergic neurons and noradrenergic neurons in an intrinsic cardiac ganglion and the stellate ganglia, respectively, that project to the sinoatrial node. We also report that the heart rate response to optogenetic versus electrical stimulation of the vagus nerve displays different temporal characteristics and that vagal afferents enhance parasympathetic and reduce sympathetic tone to the heart via central mechanisms. Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs.

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Mentioned in this Paper

Sinoatrial Node
Entire Autonomic Nervous System
Parasympathetic Nerve Structure
Structure of Superior Cardiac Nerve
Regulation of Biological Process
Muscle Innervation, Function
Synovial Cyst

About this Paper

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