Identification of pertussis-specific effector memory T cells in preschool children

Clinical and Vaccine Immunology : CVI
Lia G H de RondAnne-Marie Buisman

Abstract

Whooping cough remains a problem despite vaccination, and worldwide resurgence of pertussis is evident. Since cellular immunity plays a role in long-term protection against pertussis, we studied pertussis-specific T-cell responses. Around the time of the preschool acellular pertussis (aP) booster dose at 4 years of age, T-cell memory responses were compared in children who were primed during infancy with either a whole-cell pertussis (wP) or an aP vaccine. Peripheral blood mononuclear cells (PBMCs) were isolated and stimulated with pertussis vaccine antigens for 5 days. T cells were characterized by flow-based analysis of carboxyfluorescein succinimidyl ester (CFSE) dilution and CD4, CD3, CD45RA, CCR7, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α) expression. Before the aP preschool booster vaccination, both the proliferated pertussis toxin (PT)-specific CD4(+) and CD8(+) T-cell fractions (CFSE(dim)) were higher in aP- than in wP-primed children. Post-booster vaccination, more pertussis-specific CD4(+) effector memory cells (CD45RA(-) CCR7(-)) were induced in aP-primed children than in those primed with wP. The booster vaccination did not appear to significantly affect the T-cell memory subsets and functiona...Continue Reading

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Citations

Sep 9, 2015·Pathogens and Disease·Jolanda BrummelmanKingston H G Mills
Aug 6, 2015·Pathogens and Disease·Giorgio FedeleClara Maria Ausiello
Jan 4, 2017·Human Vaccines & Immunotherapeutics·Zhiyun Chen, Qiushui He
May 24, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mieszko M WilkKingston H G Mills
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Mar 16, 2017·Cold Spring Harbor Perspectives in Biology·Nicolas BurdinStanley A Plotkin
Jun 20, 2018·The Journal of Clinical Investigation·Ricardo da Silva AntunesAlessandro Sette
Jun 30, 2019·Toxins·Karen ScanlonNicholas Carbonetti

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