PMID: 7090420Jan 1, 1982Paper

Identification of phenolic acids in human urine by ion monitoring

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
J W HubbardJ K Cooper

Abstract

1. Two-different double derivatization techniques and two different g.l.c. systems were used to separate isomeric phenolic carboxylic acids in normal human urine. 2. Carboxylic acids were converted into n-butyl esters and phenolic functions into trifluoroacetic acid esters. 3. n-Butyl trifluoroacetoxybenzoates were separated by g.l.c. and detected by mass spectrometric single-ion monitoring. 4. Trifluoroacetates were hydrolysed under mild conditions, and liberated phenolic groups were subjected to flash methylation. 5. n-Butyl methoxybenzoates were separated by g.l.c. and detected by flame ionization. 6. All derivatives were identified by comparison of retention times and mass spectra with those of authentic reference standards. 7. The urine of a normal vegetarian volunteer was examined. 8. The presence of meta- and para-hydroxybenzoic acids, vanillic acid and isovanillic acid was confirmed by unambiguous techniques.

References

Aug 1, 1979·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·K K MidhaJ W Hubbard
Jun 1, 1966·The Journal of Pharmacy and Pharmacology·L G DringR T Williams
Aug 1, 1972·The Biochemical Journal·J CaldwellR T Williams
Jul 1, 1969·Environmental Research·L Fishbein, H L Falk
Jul 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Price
Mar 8, 1958·Nature·P SMITH, A M BENNETT
Mar 1, 1964·Clinica Chimica Acta; International Journal of Clinical Chemistry·W VONSTUDNITZA SJOERDSMA

Citations

Feb 1, 1985·Biomedical Mass Spectrometry·A HeindlG Spiteller

Related Concepts

Chromatography
Hydrolysis
Mass Spectrometry
Vegan Diet
Hydroxybenzoates

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