Identification of phenolic compounds by HPLC-ESI-MS/MS and antioxidant activity from Chilean propolis

Food Research International
Consuelo CastroCarla Delporte

Abstract

Propolis is a complex hive product produced by honey bees, Apis mellifera. Its composition and biological activities depend on the vegetation where hives are placed. Propolis is often used as a food supplement. The aim of this research is to determine the antioxidant properties in vitro and the phenolic composition of six propolis collected from the region of Santiago of Chile. We obtained the ethanolic extracts dry and wax free (EEPs) and studied their antioxidant properties by FRAP, ORAC-FL, ORAC-PGR and DPPH radical methods. The total phenols were quantified by a spectrophotometric method and 30 phenolic compounds were identified by HPLC-ESI-MS/MS analysis. Curacaví EEP has the highest relative abundance of caffeic acid phenylethyl ester (CAPE) while Buin EEP has the highest relative abundance of caffeic acid benzyl ester and quercetin. Both have the highest antioxidant activity in vitro in all methods used. Our research shows, for the first time, a comparative analysis of the antioxidant activity and phenolic composition of six Chilean propolis. Pinobanksin is the only phenol present in the six samples of propolis so it may be a good candidate for the standardization of propolis ethanolic extracts in the region of Santiago.

Citations

Jan 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Radmila PavlovicAnnamaria Giorgi
Jul 4, 2015·Journal of the Science of Food and Agriculture·Nélida NinaGuillermo Schmeda-Hirschmann
Oct 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nélida NinaGuillermo Schmeda-Hirschmann
Mar 28, 2019·BioMed Research International·Krzysztof SkowronEugenia Gospodarek-Komkowska
Apr 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiasen JiangFuliang Hu
May 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shengwei SunGuangling Yin
Jan 13, 2021·Antioxidants·Marianna Martinello, Franco Mutinelli
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena RatajczakJan Matysiak
Oct 13, 2021·Scientific Reports·Mădălina Maria NichitoiVasile Lavric

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