Identification of phosphorylated proteins associated with the fibroblast growth factor receptor type I during early Xenopus development

Biochemical and Biophysical Research Communications
P J RyanL L Gillespie

Abstract

Signaling through the FGF receptor (FGFR) is required for mesoderm induction in Xenopus. Some of the downstream signaling molecules implicated in this developmental process include Ras, Raf and MAP kinase. In a previous report, we demonstrated that PLC gamma 1, Grb-2, SOS and Nck were associated with activated FGFR1s in a signaling complex in Xenopus blastulae. In addition, several unidentified phosphotyrosylproteins were present in the FGFR1 complex. Here we identify three of these proteins as Ras-GAP, the p85 of P13'K and SHP2, while demonstrating that c-Src and She were not associated with the FGFR1. Furthermore, we show that three additional phosphotyrosylproteins from the FGFR1 complex specifically bound to the adaptor molecule Nck.

References

Aug 1, 1992·Mechanisms of Development·L L GillespieJ M Slack
May 21, 1992·Nature·M Whitman, D A Melton
Jan 1, 1989·Progress in Growth Factor Research·G D Paterno, L L Gillespie
Feb 16, 1995·Nature·T Pawson
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R E Friesel, T Maciag
Mar 1, 1995·Molecular and Cellular Biology·Q HuL T Williams
Feb 15, 1996·Biochemical and Biophysical Research Communications·T KitamuraM Kasuga
Jan 31, 1997·The Journal of Biological Chemistry·G Lussier, L Larose
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·M TanakaB J Mayer

❮ Previous
Next ❯

Citations

May 12, 2010·The Journal of Biological Chemistry·Irini TossidouMario Schiffer
May 29, 2007·Developmental Biology·Diane C Slusarski, Francisco Pelegri
Dec 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·José DíazGustavo Martínez-Mekler
Sep 1, 2000·The Journal of Biological Chemistry·C E AdlerB J Mayer
Sep 26, 2001·The Journal of Biological Chemistry·N KinklD Hicks
Feb 4, 2005·Endocrine Reviews·Ralph T Böttcher, Christof Niehrs
Jan 4, 2017·PloS One·Roya DerwishLaura L Gillespie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.