Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro

Phytochemistry
H ChassaigneM H Zenk

Abstract

An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC (PC, desGly-PC and iso-PC(Glu)) that were observed, novel PC and desGly-PC homologues lacking the N-terminal gamma-linked Glu were isolated from maize root extracts for the first time. Additionally the complete sequence of iso-PC3(Glu) was determined by tandem mass spectrometry. Peptides obtained in vivo and in vitro as the result of the reaction of glutathione with the enzyme phytochelatin synthase were compared. Minor forms detected from in vitro reactions include compounds with intramolecular or intermolecular disulfide bonds resulting from the oxidation of SH groups, phytochelatin homologues lacking the N-terminal gamma-linked Glu, and new PC-related peptides with a Cys-Cys motif. Since peptides lacking a gammaGlu residue could be generated as artifacts in electrospray mass spectrometry, the application of capillary electrophoresis with online electrospray mass spectrometry allowed the separation and detection...Continue Reading

References

Mar 1, 1995·The Plant Journal : for Cell and Molecular Biology·P MeuwlyW E Rauser
Feb 19, 2000·Phytochemistry·H KubotaT Maitani
Mar 11, 2000·Plant Physiology·M E SchmögerE Grill

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Citations

Jul 8, 2005·Analytical and Bioanalytical Chemistry·M H A El-ZohriH Frank
Jul 11, 2009·Analytical and Bioanalytical Chemistry·Anja BräutigamDirk Wesenberg
Apr 20, 2012·Analytical and Bioanalytical Chemistry·B Alan Wood, Jörg Feldmann
Jan 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Maria Rowena N Monton, Shigeru Terabe
Jun 12, 2012·Phytochemistry·Nanna Heinz, Birger Lindberg Møller
Mar 16, 2012·Journal of Plant Physiology·Raquel Requejo, Manuel Tena
Dec 11, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Baki B M SadiJoseph A Caruso
Aug 9, 2011·Plant, Cell & Environment·Anja BräutigamDirk Wesenberg
Mar 15, 2005·Plant Physiology and Biochemistry : PPB·Annamaria RanieriLuigi Sanità di Toppi
Jul 19, 2019·Bioinspiration & Biomimetics·Yu-Ting ChenGuangming Xie
Mar 31, 2009·Journal of Environmental Quality·Syam S AndraStephan B H Bach
Jun 28, 2008·Journal of Agricultural and Food Chemistry·Jiann-Shing WuRong-Huay Juang

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