PMID: 11605764Oct 19, 2001Paper

Identification of plant and animal glues in museum objects by GC-MS, after catalytic hydrolysis of the proteins by the use of a cation exchanger, with simultaneous separation from the carbohydrates

Fresenius' Journal of Analytical Chemistry
U Schneider, E Kenndler

Abstract

A method is described which enables the group-separation of proteinaceous binding media from vegetable glues (carbohydrates), and simultaneous hydrolysis of the proteins in mixtures of both. The mixtures of the binders are suspended in aqueous-ethanolic solvent with the H+ form of a strong cation exchanger and treated at elevated temperature in sealed vials. The polypeptides are cleaved by H+-catalysed hydrolysis. On abstraction the amino acids are transformed into the ammonium ions by the protons, and the cations are adsorbed by the exchanger resin. The amino acids are removed from solution in this way, thus suppressing interfering reactions with other binders, e.g. humin formation with carbohydrates. Clear and colourless solutions were obtained with all mixtures of vegetable and animal glues. Two fractions can be obtained after separation of the solid resin from the liquid supernatant - the resin fraction with the adsorbed amino acids, and the aqueous-ethanolic solution with the carbohydrates. In each of these fractions the two classes of binder can be identified separately by GC-MS; this avoids the occurrence of unresolved GC peaks and superimposed mass spectra. The method has been used to identify the binder found between f...Continue Reading

Citations

Sep 19, 2002·Journal of Chromatography. a·Maria Perla ColombinAlessia Carmignani
Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Jun 25, 2008·Analytica Chimica Acta·María Teresa Doménech-Carbó
Sep 1, 2004·Journal of Separation Science·Maria Perla Colombini, Francesca Modugno
Oct 22, 2011·Analytical Chemistry·Sophie DallongevilleCaroline Tokarski
Mar 29, 2002·Journal of Mass Spectrometry : JMS
Mar 6, 2008·Journal of Agricultural and Food Chemistry·V T P VinodU V R Vijaya Saradhi

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