Identification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors

Journal of Virology
Craig Graham WebsterMarilyne Uzest

Abstract

Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a noncirculative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphid Acyrthosiphon pisum and the green peach aphid Myzus persicae, in the acrostyle, an organ earlier shown to harbor receptors of a noncirculative virus. A peptide motif present at the C termini of both Stylin-01 and Stylin-02 is readily accessible all over the surface of the acrostyle. Competition for in vitro binding to the acrostyle was observed between an antibody targeting this peptide and the helper component protein P2 of Cauliflower mosaic virus Furthermore, silencing t...Continue Reading

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Citations

Jun 10, 2020·Journal of Economic Entomology·Frederic FrancisEmilie Bosquee
Aug 6, 2020·Viruses·Cecilia A Prator, Rodrigo P P Almeida
Aug 1, 2020·Frontiers in Plant Science·Anna KolliopoulouLuc Swevers
Jul 28, 2020·Viruses·Kiran R GadhaveRajagopalbabu Srinivasan
Nov 5, 2019·Biotechnology Advances·Shaoshuai LiuKarl-Heinz Kogel
Dec 6, 2020·Applied and Environmental Microbiology·Katherine M ElstonJeffrey E Barrick
Dec 18, 2020·Frontiers in Plant Science·Aamir LalSukchan Lee
Feb 29, 2020·Insect Biochemistry and Molecular Biology·Rosanna GiordanoUNKNOWN Soybean aphid research community
Apr 3, 2021·Mitochondrial DNA. Part B, Resources·Ruichang NiuShuai Zhang
Apr 25, 2019·Insect Biochemistry and Molecular Biology·G Le TrionnaireD Tagu
Sep 4, 2021·Pest Management Science·Wikum H JayasingheMidatharahally N Maruthi
Dec 8, 2021·Nature Communications·Wikum H JayasingheChikara Masuta

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Datasets Mentioned

BETA
JX993913
MG188739
MG188743

Methods Mentioned

BETA
Transmission electron microscopy
dot blot
electron microscopy
dissecting
PCR

Software Mentioned

MUSCLE
Stylet +
LinRegPCR
Coffee
TREEDYN
OrthoMCL
fr
PhyML
EPG Systems
CuticleDB

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