Identification of plasma proteases inhibited by Manduca sexta serpin-4 and serpin-5 and their association with components of the prophenol oxidase activation pathway.

The Journal of Biological Chemistry
Youren TongMichael R Kanost

Abstract

One innate immune response pathway of insects is a serine protease cascade that activates prophenol oxidase (pro-PO) in plasma. However, details of this pathway are not well understood, including the number and order of proteases involved. Protease inhibitors from the serpin superfamily appear to regulate the proteases in the pathway. Manduca sexta serpin-4 and serpin-5 suppress pro-PO activation in plasma, apparently by inhibiting proteases upstream of the direct activator of pro-PO. To identify plasma proteases inhibited by these serpins, we used immunoaffinity chromatography with serpin antibodies to isolate serpin-protease complexes that formed after activation of the cascade by exposure of plasma to bacteria or lipopolysaccharide. Covalent complexes of serpin-4 with hemolymph proteases HP-1 and HP-6 appeared in plasma activated by Gram-positive or Gram-negative bacteria, whereas serpin-4 complexes with HP-21 and two unidentified proteases were unique to plasma treated with Gram-positive bacteria. HP-1 and HP-6 were also identified as target proteases of serpin-5, forming covalent complexes after bacterial activation of the cascade. These results suggest that HP-1 and HP-6 may be components of the pro-PO activation pathway,...Continue Reading

References

Jun 7, 1996·The Journal of Biological Chemistry·H YoshidaM Ashida
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Feb 25, 1998·Insect Biochemistry and Molecular Biology·H JiangM R Kanost
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·H JiangM R Kanost
Feb 2, 1999·Insect Molecular Biology·H JiangM R Kanost
Jun 16, 1999·Structure·J LiE J Goldsmith
Mar 4, 2000·Insect Biochemistry and Molecular Biology·H Jiang, M R Kanost
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S ThielJ C Jensenius
Aug 10, 2000·Protein Science : a Publication of the Protein Society·P R ElliottD A Lomas
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·T WernerD Hultmark
Jun 21, 2001·Immunological Reviews·G J ArlaudJ C Fontecilla-Camps
Jun 21, 2001·Immunological Reviews·M Matsushita, T Fujita
Jun 23, 2001·Advances in Experimental Medicine and Biology·H JiangM R Kanost
Jun 23, 2001·Advances in Experimental Medicine and Biology·M R KanostY Zhu
Jun 23, 2001·Advances in Experimental Medicine and Biology·A J Nappi, E Vass
Jul 6, 2001·Insect Biochemistry and Molecular Biology·H GanM R Kanost
Apr 16, 2002·Science·Ruslan Medzhitov, Charles A Janeway
Jul 6, 2002·Science·Petros LigoxygakisJean-Marc Reichhart
Sep 13, 2002·Insect Biochemistry and Molecular Biology·X-Q YuM R Kanost
Dec 12, 2002·Chemical Reviews·Peter G W Gettins
Jan 22, 2003·Insect Biochemistry and Molecular Biology·Xiao-Qiang YuMichael R Kanost
Feb 20, 2003·Developmental and Comparative Immunology·Xiao-Qiang Yu, Michael R Kanost
Jun 18, 2004·Immunological Reviews·Michael R KanostXiao-Qiang Yu

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Citations

Apr 10, 2013·Insect Biochemistry and Molecular Biology·Chansak SuwanchaichindaMichael R Kanost
Jun 3, 2009·The Journal of Biological Chemistry·Chunju AnMichael R Kanost
Sep 11, 2012·Fish & Shellfish Immunology·Piti AmparyupAnchalee Tassanakajon
Feb 22, 2012·Insect Biochemistry and Molecular Biology·Naoyuki YonemuraKazuei Mita
Sep 10, 2010·Insect Biochemistry and Molecular Biology·Joon Ha LeeIn Hee Lee
Oct 20, 2009·Developmental and Comparative Immunology·Xiang-Jun RaoXiao-Qiang Yu
Jun 2, 2009·Insect Biochemistry and Molecular Biology·Prashant S PyatiJohn A Gatehouse
Jan 24, 2009·Insect Biochemistry and Molecular Biology·Erjun LingXiao-Qiang Yu
Jul 16, 2008·Insect Biochemistry and Molecular Biology·Yang Wang, Haobo Jiang
Dec 22, 2015·Current Opinion in Insect Science·Michael R Kanost, Haobo Jiang
Apr 25, 2007·Developmental and Comparative Immunology·Thanawat SriphaijitSaengchan Senapin
Aug 15, 2015·Developmental and Comparative Immunology·Pakkakul SangsuriyaPiti Amparyup
Jan 13, 2015·The Journal of General Virology·Daniel M P Ardisson-AraujoRollie J Clem
Nov 3, 2009·Proteomics·Ladawan WasinpiyamongkolDorothée Missé
May 16, 2006·Immunobiology·Pikul JiravanichpaisalKenneth Söderhäll
Nov 1, 2005·Biochemical and Biophysical Research Communications·Casimiro Castillejo-López, Udo Häcker
Oct 6, 2005·Insect Biochemistry and Molecular Biology·Junsuo S LiJianyong Li
Apr 17, 2016·Insect Biochemistry and Molecular Biology·Junlan LiZhiqiang Lu
Oct 16, 2012·Insect Biochemistry and Molecular Biology·Jayne M ChristenMichael R Kanost
Aug 8, 2006·Immunity·Jenny BanghamBruno Lemaitre
Jan 20, 2009·Genomics·Zhen ZouHaobo Jiang
Sep 8, 2016·Seminars in Cell & Developmental Biology·David A MeekinsKristin Michel
Feb 28, 2015·Developmental and Comparative Immunology·Dongran LiuChaoliang Liu
Sep 2, 2009·Developmental and Comparative Immunology·Jerome F La PeyreRichard K Cooper

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