Abstract
Plasmacytoid pre-dendritic cells (pDCs) are able to prime and polarize naive T-cells, while also having an important effector function in antiviral immunity through the rapid and robust production of interferon-alpha. The main setback of pDCs investigation is the rarity and ex vivo fragility of these cells. Relative simple, reliable, and accurate methods for phenotypic analysis and functional studies of pDCs without isolation would be a great deal of interest. Fresh whole blood samples were analyzed by two-color and one-color flow cytometric pDC-identification assays. The changes in the surface expression of CD62L and HLA-DQ on pDCs in whole blood samples after 24-h treatment with imiquimod, a toll-like receptor 7 agonist, were analyzed. Our data demonstrate that the identification of pDCs in peripheral blood samples can be achieved by using only one fluorescent channel for blood dendritic cell antigen (BDCA)-4 staining combined with the light scatter parameters, thus leaving the other channels open for further phenotypic and/or functional analysis. Recently, several lines of evidence supported the involvement of pDCs in the development of several human diseases, so our new one-color identification approach may provide a useful...Continue Reading
References
Jun 12, 1999·Science·F P SiegalY J Liu
Jul 30, 1999·Nature Medicine·M CellaM Colonna
Mar 23, 2001·Nature Immunology·M CellaM Colonna
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A DzionekJ Schmitz
Jul 13, 2001·European Journal of Immunology·A KrugG Hartmann
Sep 19, 2001·The Journal of Experimental Medicine·N KadowakiY J Liu
Jan 29, 2002·Nature Immunology·Hiroaki HemmiShizuo Akira
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veit HornungGunther Hartmann
Dec 14, 2002·Human Immunology·Simon RothenfusserGunther Hartmann
Feb 27, 2003·Immunology·Kenichirou TajimaShirou Fukuhara
Sep 18, 2003·Arthritis and Rheumatism·Stina BlombergLars Rönnblom
Feb 1, 1958·Acta Haematologica·K LENNERT, W REMMELE
Mar 18, 2005·Annual Review of Immunology·Yong-Jun Liu
Apr 9, 2005·Nature·Kenya HondaTadatsugu Taniguchi
Jul 16, 2005·Journal of the American Academy of Dermatology·Natalija Novak, Thomas Bieber
Feb 4, 2006·Journal of Immunological Methods·James A IdaPatrick A J Haslett
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Hamida Hammad, Bart N Lambrecht
Oct 20, 2006·Cytometry. Part B, Clinical Cytometry·Pilar María Hernández-CampoAlberto Orfao
Dec 13, 2006·International Immunopharmacology·Markus J BartenJan F Gummert
Feb 3, 2007·European Journal of Immunology·Marco Colonna
Feb 27, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jeffrey H RuthAlisa E Koch
Jun 15, 2007·Journal of Leukocyte Biology·Jason D MarshallGary Van Nest
Jul 4, 2007·Experimental Gerontology·Begoña Pérez-CabezasFrancesc E Borràs
Citations
Feb 14, 2013·PloS One·Attila SzaboEva Rajnavolgyi
May 21, 2014·Immunology and Cell Biology·Attila SzaboAttila Bacsi
Dec 20, 2011·Free Radical Biology & Medicine·Kitti PazmandiAttila Bacsi
Sep 11, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·David M Duriancik, Kathleen A Hoag
Jun 9, 2009·Journal of Biophotonics·Anja Mittag, Attila Tárnok
Mar 29, 2014·Cytometry. Part B, Clinical Cytometry·Katja JohnMarkus J Barten
May 1, 2015·International Immunology·Kachiko HayashiAkiko Iwasaki
Oct 13, 2016·Traffic·Yunhao Tan, Jonathan C Kagan