PMID: 2496116May 1, 1989Paper

Identification of plasmid and Bacillus subtilis chromosomal recombination sites used for pE194 integration

Journal of Bacteriology
L A Dempsey, D Dubnau

Abstract

The plasmid pE194 (3.7 kilobases) is capable of integrating into the genome of the bacterial host Bacillus subtilis in the absence of the major homology-dependent RecE recombination system. Multiple recombination sites have been identified on both the B. subtilis chromosome and pE194 (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, Mol. Gen. Genet. 189:58-68, 1983). The B. subtilis chromosomal recombination sites were recovered by genetic cloning, and these sites were studied by nucleotide sequence analysis. Recombination had occurred between regions of short nucleotide homology (6 to 14 base pairs) as indicated by comparison of the plasmid and the host chromosome recombination sites with the crossover sites of the integration products. Recombination between the homologous sequences of the plasmid and the B. subtilis genome produced an integrated pE194 molecule which was bounded by direct repeats of the short homology. These results suggest a recombination model involving a conservative, reciprocal strand exchange between the two recombination sites. A preferred plasmid recombination site was found to occur within a 70-base-pair region which contains a GC-rich dyad symmetry element. Five of seven pE194-integrated strains anal...Continue Reading

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Citations

Jan 1, 1991·Genetica·V I Bashkirov, D J Zvingila
Sep 3, 2005·Nature Reviews. Microbiology·Christopher M Thomas, Kaare M Nielsen
Aug 4, 2018·Wiley Interdisciplinary Reviews. RNA·Radoslaw Pluta, Manuel Espinosa
May 1, 1989·Journal of Bacteriology·L A Dempsey, D A Dubnau
Oct 1, 1989·Journal of Bacteriology·M AlbanoD A Dubnau
Feb 1, 1993·Journal of Bacteriology·P G QuirkT A Krulwich
Jun 25, 2015·Microbiology Spectrum·Ana María Hernández-ArriagaRamón Díaz-Orejas
Dec 9, 2021·Microbiology and Molecular Biology Reviews : MMBR·M Pilar Garcillán-BarciaManuel Espinosa

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