Identification of plasminogen activator in osteoclasts

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
B L GrillsT J Martin

Abstract

Plasminogen activator (PA) was located in newborn rat osteoclasts using a single-cell assay. Immunohistochemistry using biotin-streptavidin-peroxidase indicated the presence of both tissue-type plasminogen activator (tPA) and urokinase (uPA) within the cytoplasm of osteoclasts isolated from newborn rat long bones. Electron microscopic immunohistochemistry using the biotin-streptavidin-colloidal gold system on L.R. Gold thin resin sections of undecalcified, newborn rat tibial metaphyseal trabecular bone identified these proteases in the lysosomal network of osteoclasts. uPA was also localized in marrow macrophage lysosomes, but tPA was not detected in these cells. The localization of these enzymes within osteoclasts may imply their involvement in bone resorption.

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Citations

Oct 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G LeloupG Vaes
Nov 1, 1994·Journal of Cellular Biochemistry·T J Martin, K W Ng
Mar 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B L GrillsA R Ward
Oct 13, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·A YoshidaT Asaga
Nov 1, 1993·The Journal of Experimental Medicine·K FullerT J Chambers
May 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J N YangC Minkin
Jan 27, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SatoJ M Delaissé
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E DaciG Carmeliet
Aug 1, 1996·Journal of Periodontology·K ChangS Kumar
Oct 1, 1996·Acta Orthopaedica Scandinavica. Supplementum·M Takagi
Jun 9, 1998·Annals of the New York Academy of Sciences·R A Chole
Sep 1, 1994·Journal of Cellular Biochemistry·P A HillM C Meikle
Apr 1, 1996·Acta Orthopaedica Scandinavica·L NordslettenA O Aasen
Nov 3, 1998·Acta Orthopaedica Scandinavica·B L Grills, J A Schuijers
Apr 1, 1994·Journal of Cellular Physiology·P G CampbellJ F Novak

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