Identification of potential blood biomarkers for early diagnosis of Alzheimer's disease through RNA sequencing analysis.

Alzheimer's Research & Therapy
Daichi ShigemizuKouichi Ozaki

Abstract

With demographic shifts toward older populations, the number of people with dementia is steadily increasing. Alzheimer's disease (AD) is the most common cause of dementia, and no curative treatment is available. The current best strategy is to delay disease progression and to practice early intervention to reduce the number of patients that ultimately develop AD. Therefore, promising novel biomarkers for early diagnosis are urgently required. To identify blood-based biomarkers for early diagnosis of AD, we performed RNA sequencing (RNA-seq) analysis of 610 blood samples, representing 271 patients with AD, 91 cognitively normal (CN) adults, and 248 subjects with mild cognitive impairment (MCI). We first estimated cell-type proportions among AD, MCI, and CN samples from the bulk RNA-seq data using CIBERSORT and then examined the differentially expressed genes (DEGs) between AD and CN samples. To gain further insight into the biological functions of the DEGs, we performed gene set enrichment analysis (GSEA) and network-based meta-analysis. In the cell-type distribution analysis, we found a significant association between the proportion of neutrophils and AD prognosis at a false discovery rate (FDR) < 0.05. Furthermore, a similar t...Continue Reading

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Methods Mentioned

BETA
RNA-seq
PCA

Software Mentioned

FastQC
subread package
CIBERSORT
R
Cutadapt
GOplot
Cytoscape
STAR
DAVID
featureCounts

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