Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing

Journal of Medical Virology
Suguru TakeuchiYoshinori Ito

Abstract

Myocarditis is an inflammatory disease of the myocardium and leads to cardiac dysfunction and heart failure. Although viral infections are considered to be the most common etiology of myocarditis, the identification of the causative virus is still challenging. Recently, next-generation sequencing (NGS) has been applied in the diagnosis of infectious diseases. The aim of the current study was to comprehensively analyze potential pathogenic microorganisms using NGS in the sera of patients with myocarditis. Twelve pediatric and five adult patients hospitalized for acute myocarditis were included. Serum samples in the acute phase were obtained and analyzed using NGS to detect pathogen-derived DNA and RNA. Viral sequence reads were detected in 7 (41%) of the 17 myocarditis patients by NGS. Among these patients, detection of Epstein-Barr virus, human parvovirus B19, torque teno virus, and respiratory syncytial virus reads by NGS was consistent with polymerase chain reaction or antigen test results in one patient each. A large number of human pegivirus reads were detected from one patient by RNA sequencing; however, its pathogenicity to human is unknown. Conversely, the number of detected virus-derived reads was small in most cases, a...Continue Reading

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Citations

Mar 4, 2020·Herz·Bernhard Maisch, Sabine Pankuweit
Oct 14, 2020·Nature Reviews. Cardiology·Carsten TschöpeSophie Van Linthout
Sep 22, 2020·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Suguru TakeuchiYoshinori Ito
Dec 12, 2020·Signal Transduction and Targeted Therapy·Weijian HangDao Wen Wang
Oct 6, 2021·Cardiovascular Research·Heinz-Peter SchultheissFelicitas Escher
Sep 7, 2020·Journal of cardiology·Jun-Ichi KawadaYoshinori Ito

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