Identification of potential prognostic biomarkers for node-negative breast tumours by proteomic analysis: a multicentric 2004 national PHRC study

International Journal of Oncology
Françoise DescotesGabriel Ricolleau

Abstract

We used a 2D-electrophoresis (2-DE) proteomic approach to identify novel biomarkers in node-negative breast cancers. This retrospective study focused on a population of patients with ductal pN0M0 tumours. A subset of patients who developed metastases and in whose tumours were found high levels of uPA and PAI-1 (metastatic relapse, MR: n=20) were compared to another subset in whom no metastatic relapse occurred and whose tumours were found to have low levels of uPA and PAI-1 (no relapse, NR: n=21). We used a 2-DE coupled with MS approach to screen cytosol fractions using two pH-gradient scales, a broad scale (3.0-11.0) and a narrower scale focussing in on a protein rich region (5.0-8.0). This study was conducted on 41 cytosol specimens analyzed in duplicate on two platforms. The differential analysis of more than 2,000 spots in 2-DE gels, obtained on the two platforms, allowed the identification of 13 proteins which were confirmed by western blotting. Two proteins, GPDA and FABP4 were down-regulated in the MR subset whereas all the others were up-regulated. An in silico analysis revealed that GMPS (GUAA), GAPDH (G3P), CFL1 (COF1) and FTL (FRIL), the most informative genes, displayed a proliferation profile (high expression in ba...Continue Reading

Citations

Dec 4, 2012·International Journal of Molecular Sciences·Rocco SavinoRosa Terracciano
Oct 19, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nicole M A WhiteGeorge M Yousef
Mar 5, 2015·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·Bashar A ZeidanRamsey I Cutress

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