Identification of potential SLA-I-restricted CTL epitopes within the M protein of porcine reproductive and respiratory syndrome virus.

Veterinary Microbiology
Chao LiangAiping Wang

Abstract

CD8+ cytotoxic T lymphocytes (CTLs), are essential for clearance of porcine reproductive and respiratory syndrome virus (PRRSV) infection and regulation of host immune responses. Identification of SLA I-restricted CD8+ CTL epitopes would facilitate PRRSV vaccine development. Here, cells isolated from peripheral blood mononuclear cells (PBMCs) of PRRSV-immunized Large White pigs (JXA1-R strain) were screened for immunodominant PRRSV-2 M protein T cell epitopes via ELISPOT assay. Of nine immunodominant epitopes detected, eight elicited significant IFN-γ secretion responses that varied among individual pigs and according to epitope. To predict which epitopes harbored potential CTL epitopes, swine leukocyte antigen (SLA) class I genes of Large White pigs were cloned and sequenced, yielding fourteen distinct SLA class I gene sequences. Based on ELISPOT and SLA genotyping results, SLA-restricted binding of the fourteen predicted class I proteins to peptides derived from the eight immunodominant epitopes were predicted in-silico. After evaluation of 42 pET-peptide-SLA-I-β2m complexes containing predicted restricted peptides, extracellular SLA class I domains and β2m, ELISA testing of 33 peptide-SLA-I-β2m complexes detected four comple...Continue Reading

References

Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Minghai ZhouGeorge F Gao
Dec 10, 2015·Annual Review of Animal Biosciences·Joan K LunneyGourapura J Renukaradhya
Jun 28, 2017·Trends in Microbiology·Taofeng DuEn-Min Zhou
Mar 8, 2019·Frontiers in Veterinary Science·Sergio Montaner-TarbesLorenzo Fraile

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