Identification of powdered Chinese herbal medicines by fluorescence microscopy, Part 1: Fluorescent characteristics of mechanical tissues, conducting tissues, and ergastic substances

Microscopy Research and Technique
Ya-Qiong WangPing Li

Abstract

The light microscope has been successfully used in identification of Chinese herbal medicines (CHMs) for more than a century. However, positive identification is not always possible. Given the popularity of fluorescence microscopy in bioanalysis, researchers dedicated to finding new ways to identify CHMs more effectively are now turning to fluorescence microscopy for authentication purposes. Some studies on distinguishing confused species from the same genus and on exploring distributions of chemicals in tissues of CHMs by fluorescence microscopy have been reported; however, no systematic investigations on fluorescent characteristics of powdered CHMs have been reported. Here, 46 samples of 16 CHMs were investigated. Specifically, the mechanical tissues including stone cells and fibers, the conducting tissues including three types of vessels, and ergastic substances including crystals of calcium oxalate and secretions, in various powdered CHMs were investigated by both light microscope and fluorescence microscope. The results showed many microscopic features emit fluorescence that makes them easily observed, even against complex backgrounds. Under the fluorescence microscope, different microscopic features from the same powdered...Continue Reading

References

Dec 14, 2004·Journal of Ethnopharmacology·Kelvin Chan
Sep 21, 2005·Microscopy Research and Technique·Zhong-Zhen ZhaoTingguo Kang
Aug 12, 2006·Planta medica·Zhongzhen ZhaoKelvin Sze-Yin Leung
Nov 6, 2008·Microscopy Research and Technique·Dawn Tung AuZhongzhen Zhao
May 21, 2009·Microscopy Research and Technique·Dan XueCheng Peng
Nov 10, 2009·Journal of Biomedical Optics·Mark C van TurnhoutJohan L van Leeuwen
Mar 11, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Werner KaminskyAnthony Michael Glazer

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