PMID: 6166530May 1, 1981Paper

Identification of prekeratin by immunofluorescence staining in the differential diagnosis of tumors

Human Pathology
W SieinskiH L Ioachim

Abstract

Fibrillar proteins with a role in cellular shape and motility are present in the cytoplasm of most animal cells. They vary greatly in size, organization, and reactivity according to cell type and can be separately identified by the use of recently developed monospecific antisera and indirect immunofluorescence staining. Prekeratin, a structural protein in the form of intermediate sized filaments is present exclusively in cells of epithelial origin. In the present study 41 human tumors of various organs and their normal tissue counterparts were reacted with prekeratin antiserum and examined by immunofluorescence staining in paraffin embedded sections. Prekeratin was identified in all epithelial cells of the squamous type, which gave the strongest staining reaction, and in smaller amounts in epithelial cells of other histologic types. Cells of lymphoid, melanic, neural, and connective tissue origin were not stained. Thus, combining the specificity of antiprekeratin sera with the selectivity of immunofluorescence staining resulted in a new method of identifying tissues that is applicable to the differential diagnosis of tumors.

References

Mar 30, 1978·Biochemical and Biophysical Research Communications·L D Lee, H P Baden
Oct 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R H GrayI A Bernstein
Dec 1, 1976·Cancer·H L IoachimE Paluch
Jan 1, 1978·American Journal of Clinical Pathology·B H Dorsett, H L Ioachim
Jan 1, 1978·Cell·R O Hynes, A T Destree
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeK Weber
Nov 18, 1975·Biochimica Et Biophysica Acta·L D LeeH P Baden
Mar 1, 1976·The Journal of Cell Biology·P Cooke
Nov 18, 1975·Biochimica Et Biophysica Acta·L N Jones

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Citations

Apr 1, 1985·Journal of Cellular Physiology·M L Steinberg, V Defendi
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·F GiangasperoG Piazza
Jan 1, 1982·Virchows Archiv. A, Pathological Anatomy and Histology·F Bejui-ThivoletM Faure
May 1, 1989·Zentralblatt für Veterinärmedizin. Reihe A·J H VosM de Neijs
Sep 1, 1984·Histopathology·H Loosli, J Hurlimann
Jun 1, 1983·Journal of Clinical Pathology·B A GustersonR L Carter
Oct 1, 1984·The British Journal of Ophthalmology·P S HiscottD McLeod
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G A BlobelI Vogt-Moykopf
Jan 1, 1987·Journal of Cancer Research and Clinical Oncology·M BrockmannK M Müller
Jan 1, 1982·Virchows Archiv. A, Pathological Anatomy and Histology·B GustersonJ Sloane
Aug 1, 1982·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MiettinenI Virtanen
Nov 1, 1985·The Journal of Urology·A A MolinoloA I Sens
Jul 1, 1987·Human Pathology·J LinderJ D Shelburne
Jan 1, 1985·Critical Reviews in Oncology/hematology·S D Bernal, R A Stahel

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