Identification of proanthocyanidins from litchi (Litchi chinensis Sonn.) pulp by LC-ESI-Q-TOF-MS and their antioxidant activity

PloS One
Qiang LvKunsong Chen

Abstract

Content of total proanthocyanidins as well as total phenolics, flavonoids, antioxidant activities were evaluated for litchi (Litchi chinensis Sonn.) pulp of 32 cultivars. One cultivar, Hemaoli, showed the highest total proanthocyanidins and total phenolics, and DPPH or ABTS radical scavenging activities. ESI-MS and NMR analysis of the Hemaoli pulp crude extracts (HPCE) showed that procyandins composed of (epi)catechin unites with degree of polymerization (DP) of 2-6 were dominant proanthocyanidins in HPCE. After the HPCE was fractionated by a Sephadex LH-20 column, 32 procyanidins were identified by LC-ESI-Q-TOF-MS in litchi pulp for the first time. Quantification of individual procyanidin in HPCE indicated that epicatechin, procyanidin B2, procyanidin C1 and A-type procyanidin trimer were the main procyanidins. The radical scavenging activities of different fractions of HPCE as well as six procyanidins standards were evaluated by both DPPH and ABTS assays. HPCE fractions showed similar antioxidant activities with those of Vc and six individual procyanidins, the IC50 of which ranged from 1.88 ± 0.01 to 2.82 ± 0.10 μg/ml for DPPH assay, and from 1.52 ± 0.17 to 2.71 ± 0.15 μg/ml for ABTS assay. Such results indicate that litchi c...Continue Reading

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Citations

Sep 8, 2015·Journal of Ethnopharmacology·Sabrin R M Ibrahim, Gamal A Mohamed
Feb 17, 2017·Journal of Agricultural and Food Chemistry·Cédric De TaeyeSonia Collin
Aug 7, 2018·PloS One·Katyuce de S FariasCarlos A Carollo
Feb 26, 2020·Chemistry & Biodiversity·Marek BunseDietmar Rolf Kammerer
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Jun 2, 2017·Environmental Science and Pollution Research International·Hoang Nam PhamMarie-Geneviève Dijoux-Franca
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Lei ZhaoZhuoyan Hu

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Methods Mentioned

BETA
NMR

Software Mentioned

Analyst ® TF
OriginPro

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