Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene-doping detection considering the presence of pseudogenes.

Animal Genetics
Teruaki TozakiShun-Ichi Nagata

Abstract

Processed pseudogenes, also known as retrocopy genes, are copies of messenger RNAs that have been reverse transcribed into DNA and inserted into the genome. In this study, we identified 62 processed pseudogene candidates as intron-less genes from whole-genome sequencing (WGS) data of Thoroughbred horses using delly structural variation software. The 62 processed pseudogene candidates were confirmed by PCR amplification of intron-less products. A total of 11 processed pseudogenes were confirmed in the genome of all 23 analysed horses, whereas three processed pseudogenes with structures of ATP11B, DPH3 and RPL17 were detected in only one of 115 horses by PCR amplification of intron-less products. Currently, most of the gene doping tests proposed in human and horse sports are adapted PCR-based methods using hydrolysis probes to detect exon/exon junctions in transgenes because the operation is simple and economical. However, when the pseudogene is present in the host genome, the PCR-based methods may have a potential risk of detecting false positives. In this study, because processed pseudogenes that exist less frequently in the horse genome may affect PCR-based transgene detection in gene-doping tests, we propose and demonstrate t...Continue Reading

References

Jul 10, 2010·Nucleic Acids Research·Ekta KhuranaMark B Gerstein
Sep 4, 2014·Mobile DNA·Haig H Kazazian
Dec 6, 2016·International Journal of Molecular Sciences·Jin XiaoFrank M You
Oct 19, 2017·Drug Testing and Analysis·Olivier SalaminNicolas Leuenberger
Jul 5, 2019·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jing YiZhao-Qun Deng
Dec 8, 2020·Open Veterinary Journal·Alexandr AimaletdinovElena Zakirova
Jun 9, 2021·Gene Therapy·Teruaki Tozaki, Natasha A Hamilton
Aug 8, 2021·Scientific Reports·Teruaki TozakiShun-Ichi Nagata

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