Identification of prostate-specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms.

Journal of Proteomics
Ákos VégváriThomas Laurell

Abstract

Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification. Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS-PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were iden...Continue Reading

References

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Citations

Sep 20, 2011·Journal of Clinical Bioinformatics·Akos VégváriGyörgy Marko-Varga
Feb 4, 2016·International Journal of Biological Macromolecules·Paresh B BhanushaliChander P Puri
Jan 3, 2012·Clinical Biochemistry·Ákos VégváriGyörgy Marko-Varga
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Tânia S C R RebeloJoão Costa-Rodrigues
Jun 1, 2010·Proteomics·Rebekah L GundryJennifer E Van Eyk
Oct 14, 2017·Expert Review of Proteomics·Lakmali Munasinghage Silva, Judith Ann Clements
Nov 16, 2019·Proteomics·Constance A SobseyChristoph H Borchers
Apr 27, 2010·Chemical Reviews·Akos Végvári, György Marko-Varga

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