IDENTIFICATION OF PROTEASOME ALPHA6 SUBUNIT ASSOCIATED WITH DELTAMETHRIN RESISTANCE IN Drosophila melanogaster Kc CELLS

Archives of Insect Biochemistry and Physiology
Junli HuLuogen Cheng

Abstract

Differential expression of the proteasome alpha6 (prosalpha6) was previously reported between Plutella xylostella strains that are resistant or susceptible to the pesticide deltamethrin (DM). This finding indicated that the prosalpha6 may be involved in DM resistance. In this article, qPCR analysis revealed that the prosalpha6 was also significantly upregulated in Drosophila Kc cells treated with DM. To better understand the contribution of prosalpha6 in DM resistance, RNA interference, heterologous expression, and a proteasome inhibitor (MG-132) were used. MG-132 was used to suppress proteasomal activity, and the dsRNA was designed to block the function of prosalpha6. The results indicated that both MG-132 and prosalpha6 knockdown decreased the cellular viability following DM treatment. Prosalpha6 was cloned and transfected into Drosophila Kc cells. The result showed that overexpression of prosalpha6 in Drosophila Kc cells conferred some protection against DM. Taken together, our results indicate that prosalpha6 is involved in Drosophila cells DM resistance.

References

Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Feb 5, 2000·The Biochemical Journal·M G PatonJ Hemingway
Aug 22, 2001·Chemistry & Biology·A F Kisselev, A L Goldberg
Jan 29, 2002·Toxicology·David M SoderlundMyra L Weiner
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
Sep 28, 2002·Science·I DenholmM S Williamson
Oct 5, 2002·Science·Janet HemingwayJohn Vontas
Mar 21, 2003·Biochemical and Biophysical Research Communications·Shigetsugu Hatakeyama, Kei-ichi I Nakayama
Aug 21, 2003·Ecotoxicology and Environmental Safety·Iqbal SayeedSheikh Raisuddin
Sep 30, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Angelika M Burger, Arun K Seth
Dec 23, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andre GoyAlma M Rodriguez
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe DavidHilary Ranson
Jan 18, 2006·Annual Review of Medicine·Paul G RichardsonKenneth C Anderson
Sep 25, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Laura SmithLynn Cawkwell
Jul 11, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qinggui YangChangliang Zhu
Jun 6, 2009·Annual Review of Biochemistry·Daniel Finley
Apr 30, 2010·Trends in Cell Biology·Lynn BedfordJeroen Roelofs
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fuqiang Geng, William P Tansey

❮ Previous
Next ❯

Citations

Nov 28, 2018·Archives of Insect Biochemistry and Physiology·GuiLu Zhang, WenJun Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.