Identification of protein-coding and non-coding RNA expression profiles in CD34+ and in stromal cells in refractory anemia with ringed sideroblasts.

BMC Medical Genomics
Mariana O BarattiSara T Olalla-Saad

Abstract

Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease. In this study, gene expression profiles of CD34+ cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for non-coding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts. In C...Continue Reading

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Citations

Jan 15, 2014·Stem Cells and Development·Fernanda Marconi RoversiSara Teresinha Olalla Saad
Sep 30, 2016·Journal of Hematology & Oncology·Xinyu TianShengjun Wang
Nov 30, 2018·Journal of Cellular and Molecular Medicine·Fernanda Marconi RoversiSara Teresinha Olalla Saad
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Fernanda Marconi RoversiSara Teresinha Olalla Saad

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Datasets Mentioned

BETA
GPL9193
GSE18911

Methods Mentioned

BETA
MDS
fluorescence-activated cell sorting
FACS
Electrophoresis
Feature Extraction
PCR

Software Mentioned

Spotfire DecisionSite ®
Significance Analysis of Microarrays ( SAM )
FE
Ingenuity Pathways Analysis
LOWESS
SAM
Adobe Acrobat Reader
Significance Analysis of Microarray ( SAM )
Ingenuity Pathways Analysis ( IPA )
PRIMER3

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