Identification of protein markers for extracellular vesicle (EV) subsets in cow's milk

Journal of Proteomics
Abderrahim BenmoussaPatrick Provost

Abstract

Extracellular vesicles (EVs), like exosomes, are small membrane vesicles involved in cell-to-cell communications that modulate numerous biological processes. We previously discovered a new EV subset in milk (sedimenting at 35,000 g; 35 K) that protected its cargo (RNAs and proteins) during simulated digestion and was more enriched in microRNAs than exosomes (sedimenting at 100 K). Here, we used LC-MS/MS to push further the comparison between these two pellets. Commonly used EV markers were not differentially enriched between the pellets, questioning their use with cow's milk EVs. Similarly, the majority of the quantified proteins were equally enriched between the two pellets. Nevertheless, 20 proteins were specific to 35 K, while 41 were specifically enriched in 100 K (p < 0.05), suggesting their potential use as specific markers. Loaded with these proteins, the EVs in these pellets might regulate translation, proliferation and cell survival for 35 K, and metabolism, extracellular matrix turnover and immunity for 100 K. This approach also brought new insights into milk EV-associated integrins and their possible role in specifically targeting recipient cell types. These findings may help better discriminate between milk EVs, imp...Continue Reading

Citations

Sep 17, 2020·International Journal of Molecular Sciences·Sergey SedykhGeorgy Nevinsky
Jan 4, 2019·Journal of Translational Medicine·Bodo C Melnik, Gerd Schmitz
Mar 2, 2021·Frontiers in Immunology·Laura E CarrLaxmi Yeruva
Mar 7, 2021·International Journal of Molecular Sciences·Natalie TurnerMurray D Mitchell
Nov 3, 2020·Journal of Agricultural and Food Chemistry·Wenyan ChenGanglong Yang
Sep 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jonas M NikoloffPetra S Dittrich

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

The International Journal of Biochemistry & Cell Biology
Claudia Bang, Thomas Thum
International Journal of Molecular Sciences
Uyen Thi Trang ThanTony Parker
© 2022 Meta ULC. All rights reserved