Identification of proteinase 3 as the major caseinolytic activity in acute human wound fluid

The Journal of Investigative Dermatology
Y HeF Grinnell

Abstract

Wound fluid contains several proteinases that are important in the repair process. In this study, we analyzed caseinolytic activity in wound fluid obtained from acute (burn) wounds. Caseinolytic activity in wound fluid increased markedly 2 d after injury and appeared on casein zymographs as a series of bands or a smear ranging from 30 to 100 kDa. Most of the enzyme activity was inhibited by the synthetic human neutrophil elastase inhibitor MDL 27,367 but not by the naturally occurring inhibitor of elastase, human secretory leukoproteinase inhibitor. Fractionation of wound fluid indicated that a single enzyme accounted for approximately 80% of the caseinolytic activity. This enzyme degraded the elastase substrate methoxysuccinyl-ala-ala-pro-val-p-nitroanilide at a slow rate. The above findings suggested that the enzyme responsible for caseinolytic activity might be proteinase 3, an elastase-related enzyme whose physiologic functions are poorly understood. Consistent with the above possibility, we found that monoclonal antibodies against proteinase 3 removed caseinolytic activity from wound fluid, and that purified proteinase 3 had a similar caseinolytic profile and inhibitor sensitivity to burn fluid.

References

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Citations

Feb 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christina L Thomas-VirnigB Lynn Allen-Hoffmann
May 1, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Andrea HeinzleEva Sigl
Dec 21, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rachael L McInnesRyan Moseley
Jun 19, 2013·The International Journal of Lower Extremity Wounds·Markus W LöfflerStefan Beckert
Jan 13, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·D R Yager, B C Nwomeh
Aug 17, 2018·BioMed Research International·Qiwei LiuGeorgios N Stamatas
May 14, 1998·The Journal of Investigative Dermatology·F GrinnellW C Parks
Jul 10, 2003·Gastroenterology·Emiko MizoguchiAtsushi Mizoguchi

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