PMID: 9171094Jun 15, 1997Paper

Identification of proteins binding specifically to the 3'-untranslated region of granulocyte/macrophage-colony stimulating factor mRNA

Nucleic Acids Research
V E BichselM Bickel

Abstract

The 3'-untranslated region of granulocyte/macrophage colony-stimulating factor (GM-CSF) mRNA contributes to the post-transcriptional regulation of gene expression. Degradation is partly mediated by adenosine- uridine-rich sequence elements (ARE), which serve as binding sites for specific proteins. Stabilization of RNA by phytohemagglutinin and concanavalin A treatment is dependent on regulatory sequence elements upstream of ARE. We have performed northwestern blot and filter binding assays using cell extracts and RNA sequences containing or lacking ARE. Murine and human T cell extracts (EL-4 and Jurkat) yielded two specific proteins of 93 and 94 kDa, respectively, that were binding to sequences upstream of ARE. Within this region, the human and murine RNA do not share any obvious sequence identity, yet both are target sites for the binding proteins. The smallest RNA fragments protected by the proteins from RNase A digestion, were 44 in the murine, and 38 ribonucleotides long in the human sequence. The binding activity of the 94 kDa protein derived from human Jurkat cells could be enhanced by phytohemagglutinin. The interaction with regulatory mRNA sequences and the responsiveness to phytohemagglutinin suggests that the proteins...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M BickelR B Cohen
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Mar 1, 1996·Nucleic Acids Research·J Hua, V Paetkau

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Citations

Mar 28, 2012·Molecules and Cells·Sang-Yun ChoKook-Hyung Kim
Jun 8, 2004·Nucleic Acids Research·Jérôme DelaunayOunissa Aït-Ahmed
Apr 27, 2004·Periodontology 2000·Nicolas Delaleu, Matthias Bickel
Nov 5, 2013·The American Journal of Pathology·Annemarie E van NieuwenhuijzeIan P Wicks
Dec 14, 2001·The Journal of Biological Chemistry·Tadepalli Adilakshmi, Roney O Laine

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