Identification of Proteins Implicated in the Increased Heart Rate in ShenSongYangXin-Treated Bradycardia Rabbits by iTRAQ-Based Quantitative Proteomics

Evidence-based Complementary and Alternative Medicine : ECAM
Zhouying LiuJielin Pu

Abstract

The present study tries to identify proteins implicated in bradycardia rabbits in hearts after ShenSongYangXin (SSYX, a traditional Chinese medicine) treatment. Eighteen adult rabbits were randomly assigned to three groups: sham, model, and SSYX treatment groups. Heart rate was recorded in rabbits and proteins were isolated from ventricular muscle. We used isobaric tags for elative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to identify altered proteins after SSYX treatment. The heart rate decreased after six weeks due to the injury of the sinoatrial node in the model group. This effect was partially reversed by 4-week SSYX treatment. A total of a2988 proteins were quantified by performing the iTRAQ-based experiments. Of these, 86 proteins were differentially expressed according to our criteria (42 upregulated and 44 downregulated). The identification of key proteins implicated in the treatment of bradycardia could serve as a foundation to better understand and further explore the molecular mechanism of SSYX treatment.

References

Mar 25, 2005·Journal of Thrombosis and Thrombolysis·Thomas D Christensen
Oct 26, 2005·Journal of Molecular and Cellular Cardiology·Guillaume LamiraultMarja Steenman
Oct 6, 2006·Journal of Molecular and Cellular Cardiology·Luisa NanniGerolamo Lanfranchi
Oct 10, 2009·Nature Protocols·Mariusz R WieckowskiPaolo Pinton
Oct 4, 2011·Journal of Cardiac Failure·David LinUNKNOWN NCE CECR PROOF Centre of Excellence
Feb 15, 2013·Cardiovascular Research·Andriy E BelevychSandor Györke
Dec 5, 2014·BMC Genomics·Jonathan D StallingsJaques Reifman
Dec 20, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Zhouying LiuJielin Pu

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