Identification of proteins in pooled human follicular fluid which suppress follicular response to gonadotropins

The Journal of Clinical Endocrinology and Metabolism
G S diZeregaO R Kling

Abstract

To evaluate the role of nonsteroidal, follicular fluid proteins in folliculogenesis, the 10-55% saturated ammonium sulfate fraction of pooled human follicular fluid was dialyzed against 0.025 M Tris/HCl (pH 7.5) using 10,000 molecular weight exclusion membranes, then passed through agarose immobilized textile dye. Activity was determined by test fraction inhibition of human menopausal gonadotropin (2 U human LH/FSH . day), induced ovarian weight, and serum estradiol increase in hypophysectomized, diethylstilbesterol-treated, 25-day-old female rats. Specific inhibition (89 +/- 6.8% SEM) of ovarian weight increase was found in the material (2 ml) eluted from an Orange A column with KCl (1.5 M, pH 6.8). Inhibitory activity of the Orange A-bound material, which eluted through a standardized Sephadex G-50 column, corresponded to a molecular weight of 13,000-25,000. Isoelectric focusing on a Sephadex G-15 support bed or ampholyte displacement chromatography of Orange A bound material demonstrated inhibitory activity at pH 3.5-4.5 and 6.5-7.0. No demonstrable activity was found in similar fractions eluted through a Concanavalin A-Sepharose 4B column before or after addition of alpha-methyl-D-mannoside (2 M, pH 7). When active fraction...Continue Reading

Citations

Feb 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·D W RichardsR P Marrs
Aug 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·A PellicerF Naftolin
Oct 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M P DiamondA C Wentz
Jan 1, 1987·Journal of Steroid Biochemistry·G S DiZeregaG Westhof
Jun 28, 2000·Domestic Animal Endocrinology·M A DriancourtT Magallon
May 16, 2001·Biochemical and Biophysical Research Communications·B SaidD C Matsumoto
Dec 1, 1985·Journal of Endocrinological Investigation·V O CabreraO Mateo De Acosta
Feb 1, 1986·Clinics in Endocrinology and Metabolism·S A Tonetta, G S diZerega
Sep 1, 1991·The Anatomical Record·N AhmadG S diZerega
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Jan 1, 1986·Archives of Andrology·K FujimoriG S Dizerega
Feb 1, 1996·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·F Scheele, J Schoemaker
Dec 1, 1989·Asia-Oceania Journal of Obstetrics and Gynaecology·K MiyamuraT Kigawa
Oct 1, 1989·Journal of Steroid Biochemistry·P FranchimontJ M Jaspar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.