Identification of proteins in renaissance paintings by proteomics

Analytical Chemistry
Caroline TokarskiCécile Cren-Olivé

Abstract

The presented work proposes a new methodology based on proteomics techniques to identify proteins in old art paintings. The main challenging tasks of this work were (i) to find appropriate conditions for extracting proteins from the binding media without protein hydrolysis in amino acids and (ii) to develop analytical methods adapted to the small sample quantity available. Starting from microsamples of painting models (ovalbumin-based, which is the major egg white protein, and egg-based paintings), multiple extraction solutions (HCl, HCOOH, NH3, NaOH) and conditions (ultrasonic bath, mortar and pestle, grinding resin) were evaluated. The best results were obtained using a commercial kit including a synthetic resin, mortar and pestle to grind the sample in an aqueous solution acidified with trifluoroacetic acid at 1% with additional multiple steps of ultrasonic baths. The resulting supernatant was analyzed by MALDI-TOF in linear mode to verify the efficiency of the extraction solution. An enzymatic hydrolysis step was also performed for protein identification; the peptide mixture was analyzed by nanoLC/nanoESI/Q-q-TOF MS/MS with an adapted chromatographic run for the low sample quantity. Finally, the developed methodology was su...Continue Reading

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 27, 2004·Analytical and Bioanalytical Chemistry·P VandenabeeleL J Moens
Sep 1, 2004·Journal of Separation Science·Maria Perla Colombini, Francesca Modugno

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Citations

Mar 7, 2007·Analytical and Bioanalytical Chemistry·Stepanka KuckovaMilan Kodicek
Jul 3, 2007·Analytical and Bioanalytical Chemistry·Austin NevinRinaldo Cubeddu
Apr 15, 2008·Analytical and Bioanalytical Chemistry·Marek Trojanowicz
Oct 7, 2009·Analytical and Bioanalytical Chemistry·Gabriella LeoLeila Birolo
Oct 5, 2010·Analytical and Bioanalytical Chemistry·Sophie DallongevilleCaroline Tokarski
Jun 4, 2011·Analytical and Bioanalytical Chemistry·Erika RibechiniMaria Perla Colombini
May 16, 2013·Journal of Biosciences·Michela GambinoFabio Forlani
Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Jan 10, 2012·Journal of Mass Spectrometry : JMS·S CrottiC Barbante
Sep 25, 2015·Analytical Chemistry·Roberto VinciguerraLeila Birolo
Aug 26, 2016·Analytical and Bioanalytical Chemistry·Cosima Damiana CalvanoLuigia Sabbatini
Dec 17, 2008·Journal of Chromatography. a·Alessia FarinazzoPier Giorgio Righetti
Mar 28, 2018·Nature Ecology & Evolution·Jessica HendyMatthew J Collins
Apr 1, 2018·Angewandte Chemie·Meaghan MackieEnrico Cappellini
Jun 7, 2018·Rapid Communications in Mass Spectrometry : RCM·Kristen E Watts, Anthony F Lagalante
Oct 3, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jincui GuBing Wang
Jun 29, 2013·The Analyst·Daniel P KirbyT Rose Holdcraft
May 31, 2019·Expert Review of Proteomics·Pier Giorgio RighettiAlfonsina D'Amato
May 9, 2018·Applied Microbiology and Biotechnology·Maria Gabriella GiuffridaEnrica Pessione
Jul 4, 2018·Scientific Reports·Clodoaldo RoldánManuel Porcar
May 23, 2008·Analytical Chemistry·Caroline SolazzoCaroline Tokarski
Jul 31, 2007·Journal of Agricultural and Food Chemistry·Lars NilssonBjörn Bergenståhl
Oct 22, 2011·Analytical Chemistry·Sophie DallongevilleCaroline Tokarski
Jun 25, 2008·Analytica Chimica Acta·María Teresa Doménech-Carbó
Dec 7, 2021·Mass Spectrometry Reviews·Marek SmoluchJerzy Silberring

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