Identification of proteins of Francisella tularensis induced during growth in macrophages and cloning of the gene encoding a prominently induced 23-kilodalton protein.

Infection and Immunity
Igor GolovliovAnders Sjöstedt

Abstract

The adaptation of facultative intracellular bacteria to host macrophages involves regulation of the synthesis of bacterial proteins. We analyzed the protein synthesis of Francisella tularensis LVS growing intracellularly in the macrophage-like murine cell line J774 and extracellularly in culture medium. After pulse-labeling with [35S] methionine and separation by one- and two-dimensional polyacrylamide gel electrophoresis, induction of a few proteins during intracellular growth was demonstrated. One of them, a 23-kDa protein, was prominently induced in the macrophages and also when extracellularly growing F. tularensis was exposed to hydrogen peroxide. After isolation of the 23-kDa protein from a preparative two-dimensional gel, a 22-amino-acid N-terminal peptide and two peptides obtained by trypsin digestion were sequenced. Based on the sequences, degenerate oligonucleotides were constructed for use as primers in a PCR. Hybridization of amplified DNA to XbaI-digested LVS DNA identified the gene of the 23-kDa protein in a 1.3-kb DNA fragment. Nucleotide sequence analysis revealed an open reading frame encoding a putative protein of a calculated molecular mass of 22.2 kDa. The open reading frame was preceded by a sequence typica...Continue Reading

References

Sep 1, 1991·Infection and Immunity·D B Young, T R Garbe
Sep 1, 1991·Infection and Immunity·A H FortierC A Nacy
Sep 1, 1991·Infection and Immunity·L D AnthonyF E Nano
May 11, 1990·Science·N A Buchmeier, F Heffron
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G A DabiriF S Southwick
Feb 1, 1986·Infection and Immunity·T J HolzerB R Andersen
Apr 1, 1987·Journal of Clinical Microbiology·G SandströmH Wolf-Watz
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R W MorganB N Ames
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jul 1, 1995·The Journal of Clinical Investigation·B Y Lee, M A Horwitz
May 1, 1994·Journal of Leukocyte Biology·M R Alavi, L F Affronti
Jun 1, 1994·Infection and Immunity·D A DrevetsP A Campbell
Apr 1, 1993·Infection and Immunity·Y Abu KwaikN C Engleberg
Mar 1, 1961·The Journal of Infectious Diseases·J MERRIOTTC M DOWNS

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Citations

Oct 5, 2002·Clinical Microbiology Reviews·Jill EllisRichard W Titball
Jan 7, 2003·Applied and Environmental Microbiology·Hadi AbdMats Forsman
Sep 18, 2004·Journal of Bacteriology·Francis E NanoKaren L Elkins
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catharine M Bosio, Steven W Dow
May 18, 2006·Annual Review of Microbiology·Molly K McLendonLee-Ann H Allen
Feb 5, 2008·Applied and Environmental Microbiology·Joseph HorzempaGerard J Nau
Feb 6, 2008·Cellular Microbiology·Malin VonkavaaraSvenja Stöven
Jan 2, 2010·Journal of Aquatic Animal Health·Esteban SotoJohn P Hawke
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Roger D PechousThomas C Zahrt
Oct 24, 2002·FEMS Microbiology Letters·Catherine G GrayFrancis E Nano
Feb 13, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T SkottmanS Nikkari
Aug 8, 2009·Future Microbiology·Karin L MeibomAlain Charbit
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan PukatzkiJohn J Mekalanos
Feb 24, 2007·FEMS Immunology and Medical Microbiology·Kate F GriffinRichard W Titball
Apr 23, 2004·Infection and Immunity·Horacio GilDavid G Thanassi
Sep 24, 2008·Journal of Medical Microbiology·Mark I RichardsPetra C F Oyston
Dec 2, 2009·Applied and Environmental Microbiology·Jeffrey J MargolisDenise M Monack
May 19, 2010·Infection and Immunity·Monika K AhlundSvenja Stöven
Oct 19, 2012·Future Microbiology·Elodie RamondAlain Charbit
Nov 20, 2004·Nature Reviews. Microbiology·Petra C F OystonRichard W Titball
Jun 11, 2005·Environmental Microbiology·Hadi AbdGunnar Sandström
Feb 18, 2010·Infection and Immunity·Esteban SotoJohn P Hawke

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