Identification of proteins of the 40 S ribosomal subunit involved in interaction with initiation factor eIF-2 in the quaternary initiation complex by means of monospecific antibodies

FEBS Letters
U A BommerH Bielka

Abstract

Monospecific polyclonal antibodies against seven proteins of the 40 S subunit of rat liver ribosomes were used to identify ribosomal proteins involved in interaction with initiation factor eIF-2 in the quaternary initiation complex [eIF-2 X GMPPCP X [3H]Met-tRNAf X 40 S ribosomal subunit]. Dimeric immune complexes of 40 S subunits mediated by antibodies against ribosomal proteins S3a, S13/16, S19 and S24 were found to be unable to bind the ternary initiation complex [eIF-2 X GMPPCP X [3H]Met-tRNAf]. In contrast, 40 S dimers mediated by antibodies against proteins S2, S3 and S17 were found to bind the ternary complex. Therefore, from the ribosomal proteins tested, only proteins S3a, S13/16, S19 and S24 are concluded to be involved in eIF-2 binding to the 40 S subunit.

References

Jan 16, 1979·Molecular & General Genetics : MGG·E H McConkeyI G Wool
Aug 13, 1988·Lancet·J BommerT Ziegler
Jan 1, 1985·Progress in Nucleic Acid Research and Molecular Biology·H Bielka

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Citations

Dec 27, 2006·Cell Research·Allison N Martin, Yong Li
Mar 17, 2004·Genetics in Medicine : Official Journal of the American College of Medical Genetics·James E SylvesterThomas W O'Brien
May 17, 2011·Biochemistry. Biokhimii︠a︡·E A Stolboushkina, M B Garber
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Dec 14, 2006·Blood·Johan Flygare, Stefan Karlsson
Feb 17, 2021·Plant Physiology·Muhammad UzairXueyong Li
Mar 5, 2021·Nucleic Acids Research·Isabel S Naarmann-de VriesAntje Ostareck-Lederer

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