Identification of proteolipid from an extremely halophilic archaeon Halobacterium salinarum as an N,N'-dicyclohexyl-carbodiimide binding subunit of ATP synthase

Archives of Biochemistry and Biophysics
K IharaY Mukohata

Abstract

ATP synthesis in an extremely halophilic archaeon, Halobacterium salinarum, was inhibited by N-cyclohexyl-N'-[4-(dimethylamino)-alpha-naphthyl]carbodiimide (NCD-4), a fluorescent analog of N,N'-dicyclohexylcarbodiimide (DCCD). By tracing the fluorescent signal, a hydrophobic 8-kDa protein (proteolipid) was purified from the halobacterial membrane as one of the most DCCD-reactive proteins and its N-terminal amino acid sequence was determined. The gene encoding the proteolipid was found in the region upstream of the genes encoding the two major subunits of halobacterial A-type ATPase [K. Ihara and Y.Mukohata (1991) Arch. Biochem. Biophys. 286, 111-116]. Halobacterial proteolipid was more similar in size to the proteolipid of F-type ATPase than that of V-type ATPase. However, multiple amino acid sequence alignment of proteolipids showed a higher degree of relatedness between V-type and A-type ATPase proteolipids. Together with the recent finding of a triplicate proteolipid encoding gene from the methanogenic archaeon Methanococcus jannaschii [C. J. Bult et al. (1996) Science 273, 1058-1073], proteolipids from archaea seem to have diverse characteristics in comparison with those from eubacteria or from eukaryotes.

References

Sep 9, 1977·Biochemical and Biophysical Research Communications·A Matsuno-Yagi, Y Mukohata
Jan 1, 1979·Methods in Enzymology·P E Linnett, R B Beechey
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M MandelN Nelson
Aug 15, 1989·Biochemical and Biophysical Research Communications·K InatomiM Futai
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P GogartenT Oshima
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N IwabeT Miyata
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A Danon, W Stoeckenius
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
Next ❯

Citations

Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·V MüllerG Grüber
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Jun 30, 2006·Biochimica Et Biophysica Acta·Kim Lewalter, Volker Müller
Aug 1, 2006·Re/views in Environmental Science and Bio/technology·Sergiu FendrihanHelga Stan-Lotter
May 28, 2003·Extremophiles : Life Under Extreme Conditions·Astrid LinglVolker Müller
May 13, 2006·Journal of Bioenergetics and Biomembranes·Volker MüllerMichael Fritz
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·V M ZubarevaB A Feniouk
Oct 2, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Stefanie BergerCornelia U Welte
Jan 31, 2014·Chemical Reviews·Toshio AndoSimon Scheuring
Oct 7, 2010·Biophysical Journal·Ignacio CasusoSimon Scheuring

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.