Identification of Pseudomonas aeruginosa and Aspergillus fumigatus mono- and co-cultures based on volatile biomarker combinations.

Journal of Breath Research
A H NeerincxL A J Kluijtmans

Abstract

Volatile organic compound (VOC) analysis in exhaled breath is proposed as a non-invasive method to detect respiratory infections in cystic fibrosis patients. Since polymicrobial infections are common, we assessed whether we could distinguish Pseudomonas aeruginosa and Aspergillus fumigatus mono- and co-cultures using the VOC emissions. We took headspace samples of P. aeruginosa, A. fumigatus and co-cultures at 16, 24 and 48 h after inoculation, in which VOCs were identified by thermal desorption combined with gas chromatography - mass spectrometry. Using multivariate analysis by Partial Least Squares Discriminant Analysis we found distinct VOC biomarker combinations for mono- and co-cultures at each sampling time point, showing that there is an interaction between the two pathogens, with P. aeruginosa dominating the co-culture at 48 h. Furthermore, time-independent VOC biomarker combinations were also obtained to predict correct identification of P. aeruginosa and A. fumigatus in mono-culture and in co-culture. This study shows that the VOC combinations in P. aeruginosa and A. fumigatus co-microbial environment are different from those released by these pathogens in mono-culture. Using advanced data analysis techniques such as ...Continue Reading

References

Feb 1, 1991·Journal of Clinical Microbiology·A Espinel-Ingroff, T M Kerkering
Nov 24, 1999·Journal of Clinical Pathology·J R KerrR Wilson
Jan 5, 2002·International Journal of Hygiene and Environmental Health·K FiedlerS Geh
Jan 5, 2005·Journal of Bacteriology·Lauren M MashburnMarvin Whiteley
Oct 10, 2006·Advances in Microbial Physiology·Huw D WilliamsBen Ryall
Mar 1, 2008·The British Journal of General Practice : the Journal of the Royal College of General Practitioners·A Willy GraffelmanPeterhans J van den Broek
Mar 21, 2009·Medical Mycology·Stephen T ChambersMichael J Epton
Jan 5, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Christopher R SudfeldMichael P Boyle
Dec 21, 2010·Journal of Microbiological Methods·Robin Michael Statham ThornJohn Greenman
Jan 29, 2011·The European Respiratory Journal·F J GilchristW Lenney
Mar 29, 2011·Letters in Applied Microbiology·S U SavelevA De Soyza
Jan 12, 2012·Journal of Breath Research·R A BazemoreG K Podila
Jun 26, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Heather D BeanJane E Hill
Jan 12, 2013·Journal of Breath Research·Jiangjiang ZhuJane E Hill
Jul 5, 2013·Clinical Microbiology Reviews·Shneh SethiTrinad Chakraborty
Sep 26, 2013·Current Opinion in Pulmonary Medicine·Peter G MiddletonWieland Meyer
Jun 8, 2014·Eukaryotic Cell·C HeddergottJ P Latgé
Jan 31, 2015·Journal of Breath Research·Anne H NeerincxSimona M Cristescu

❮ Previous
Next ❯

Citations

Mar 25, 2017·Analytica Chimica Acta·Brigitte P GeurtsJeroen J Jansen
Jul 25, 2017·Bioanalysis·Ileana-Andreea RatiuBogusław Buszewski
Jan 27, 2018·Mycobiology·Hadeel Saeed Al-MalikiJoan W Bennett
Nov 28, 2017·Nature Communications·Layla J BarkalDavid J Beebe
Jul 21, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mohammed AshrafiArdeshir Bayat
May 5, 2018·Metabolomics : Official Journal of the Metabolomic Society·Oluwasola LawalUNKNOWN BreathDx consortium
Mar 15, 2018·Medical Mycology·Carsten SchwarzUNKNOWN ECMM/ISHAM working group Fungal respiratory infections in Cystic Fibrosis (Fri-CF)
Sep 12, 2017·Frontiers in Microbiology·Aurélien Bailly, Laure Weisskopf
Feb 21, 2018·Frontiers in Microbiology·Antonio AzzolliniJean-Luc Wolfender
Nov 9, 2019·World Journal of Microbiology & Biotechnology·Karolina Anna MielkoPiotr Młynarz
Apr 16, 2021·MSphere·Emily A Higgins KepplerHeather D Bean
May 22, 2021·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Renate KosUNKNOWN Amsterdam Mucociliary Clearance Disease (AMCD) Research Group and the Amsterdam UMC Breath Research Group
Sep 6, 2017·ACS Infectious Diseases·Waqar M AhmedStephen J Fowler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.