Identification of Pseudozyma graminicola CBS 10092 as a producer of glycolipid biosurfactants, mannosylerythritol lipids

Journal of Oleo Science
Tomotake MoritaDai Kitamoto

Abstract

A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as the sole carbon source. Based on thin layer chromatography, the extracellular glycolipids gave spots corresponding to those of mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From the structural characterization by 1H and 13C NMR, the main product was identified as 4-O-[(4'-mono-O-acetyl-2', 3'-di-O-alka(e)noyl)-beta-D-mannopyranosyl]-D-erythritol, which is a highly hydrophilic derivative of MELs known as MEL-C. According to high-performance liquid chromatography analysis, the main product, MEL-C, comprised approximately 85% of all the MELs, and the total amount reached approximately 10 g/L for 7 days. The fatty acids of the present MEL-C consisted of mainly C6, C8 and C14 acids, considerably different from those of MEL-C produced by other Pseudozyma strains such as P. antarctica and P. shanxiensis. The observed critical micelle concentration (CMC) and the surface-tension at CMC of the MEL-C were 4.0 x 10(-6) M and 24.2 mN/m, respectively, while those of MEL-A, the most intensively studied MEL, were 2.7 x 10(-6) M and 28.4...Continue Reading

References

Jul 13, 2004·Applied Microbiology and Biotechnology·U RauS Lang
Feb 25, 2005·Applied Microbiology and Biotechnology·U RauS Lang
Jun 1, 2005·Colloids and Surfaces. B, Biointerfaces·Tomohiro ImuraDai Kitamoto
Oct 20, 2005·Journal of Bioscience and Bioengineering·Dai KitamotoTadaatsu Nakahara
Dec 24, 2005·Chemistry : a European Journal·Tomohiro ImuraDai Kitamoto
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira

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Citations

Apr 16, 2013·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Aug 22, 2008·Journal of Industrial Microbiology & Biotechnology·Joseph Irudayaraj ArutchelviMukesh Doble
Sep 13, 2011·AMB Express·Randhir S MakkarIbrahim M Banat
Mar 10, 2009·Bioscience, Biotechnology, and Biochemistry·Tomotake MoritaDai Kitamoto
May 13, 2008·Journal of Oleo Science·Masaaki KonishiDai Kitamoto
Jun 16, 2010·Journal of Bioscience and Bioengineering·Masaaki KonishiYuji Hatada
Mar 10, 2015·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
May 11, 2015·Applied Microbiology and Biotechnology·Tomotake MoritaDai Kitamoto
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Masaaki KonishiDai Kitamoto
Jan 26, 2020·Applied Microbiology and Biotechnology·Ana Letícia Silva CoelhoDébora de Oliveira
Jun 22, 2018·Applied Microbiology and Biotechnology·Azusa SaikaTomotake Morita
Nov 16, 2016·Applied Microbiology and Biotechnology·Bruno Nicolau PaulinoGlaucia Maria Pastore

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