Identification of putative benzene-degrading bacteria in methanogenic enrichment cultures

Journal of Bioscience and Bioengineering
Nahoko SakaiKazuo Yamamoto

Abstract

Anaerobic benzene-degrading enrichment cultures performing methanogenesis were obtained from non-contaminated lotus field soil. Stable isotope probing with 13C benzene was used to detect the bacteria that were involved in benzene degradation. Denaturing gradient gel electrophoresis (DGGE) of fractionated samples exhibited an obvious shift of some DGGE bands to a heavier DNA fraction. An almost full-length 16S rRNA gene sequence corresponding to the DGGE band, namely Hasda-A, was obtained by constructing a clone library of the heavier fraction. The Hasda-A sequence showed only 85.1% identity with the closest identified bacterium, Syntrophus gentianae. Hasda-A may be an important bacterium involved in the initial steps of benzene degradation under methanogenic conditions, as it was the most prominent bacterium that assimilated labeled benzene early in the process of benzene degradation. A primer set was designed to quantify the gene copies of Hasda-A by quantitative PCR. Hasda-A was present at a concentration of (3.5+/-0.8) x 10(6) copies/mL and represented 8.4% of gene copies among bacteria in the enrichment culture. The enrichment culture consisted of three dominant bacterial groups: Hasda-A and both aceticlastic and hydrogenot...Continue Reading

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Citations

Apr 6, 2012·Applied Microbiology and Biotechnology·Sabine KleinsteuberCarsten Vogt
May 31, 2011·Applied and Environmental Microbiology·Graham M PumphreyJim C Spain
Jul 12, 2011·Applied and Environmental Microbiology·Dawn E HolmesDerek R Lovley
Dec 23, 2015·Applied Microbiology and Biotechnology·Maria Irianni-RennoSusan K De Long
Apr 1, 2011·Microbial Biotechnology·Carsten VogtHans-Hermann Richnow
Nov 2, 2011·Environmental Microbiology·S Jane FowlerLisa M Gieg
May 28, 2014·Current Opinion in Biotechnology·Lisa M GiegCarolina Berdugo-Clavijo
Jun 10, 2014·FEMS Microbiology Ecology·S Jane FowlerLisa M Gieg
Feb 13, 2013·FEMS Microbiology Ecology·Sven JechalkeHans H Richnow
Nov 10, 2015·Environmental Microbiology·Fei LuoElizabeth A Edwards
Sep 8, 2016·The Journal of General and Applied Microbiology·Mana NoguchiHiroaki Furumai
Dec 4, 2020·Biodegradation·Samuel C EziuzorCarsten Vogt
Aug 12, 2020·Journal of Environmental Management·Morvarid Khazraee ZamanpourKarl John Rockne
May 28, 2021·Environmental Science & Technology·Courtney R A TothElizabeth A Edwards

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